Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.

Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.

We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establi...

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Main Authors: Hirohito Haruki, Monica Rengifo Gonzalez, Kai Johnsson
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2012-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3365113?pdf=render
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spelling doaj-259dfdcff39a4f15aa49653ae0a2040b2020-11-25T01:17:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0175e3759810.1371/journal.pone.0037598Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.Hirohito HarukiMonica Rengifo GonzalezKai JohnssonWe introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins.http://europepmc.org/articles/PMC3365113?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Hirohito Haruki
Monica Rengifo Gonzalez
Kai Johnsson
spellingShingle Hirohito Haruki
Monica Rengifo Gonzalez
Kai Johnsson
Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
PLoS ONE
author_facet Hirohito Haruki
Monica Rengifo Gonzalez
Kai Johnsson
author_sort Hirohito Haruki
title Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
title_short Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
title_full Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
title_fullStr Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
title_full_unstemmed Exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
title_sort exploiting ligand-protein conjugates to monitor ligand-receptor interactions.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2012-01-01
description We introduce three assays for analyzing ligand-receptor interactions based on the specific conjugation of ligands to SNAP-tag fusion proteins. Conjugation of ligands to different SNAP-tag fusions permits the validation of suspected interactions in cell extracts and fixed cells as well as the establishment of high-throughput assays. The different assays allow the analysis of strong and weak interactions. Conversion of ligands into SNAP-tag substrates thus provides access to a powerful toolbox for the analysis of their interactions with proteins.
url http://europepmc.org/articles/PMC3365113?pdf=render
work_keys_str_mv AT hirohitoharuki exploitingligandproteinconjugatestomonitorligandreceptorinteractions
AT monicarengifogonzalez exploitingligandproteinconjugatestomonitorligandreceptorinteractions
AT kaijohnsson exploitingligandproteinconjugatestomonitorligandreceptorinteractions
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