RANK/RANKL Expression Is Induced by Cardiac Surgical Operation

• Main Authors: Jan Krejsek, Martina Koláčková, Vladimír Lonský, Manuela Trojáčková Kudlová, Jiří Manďák, Pavel Kuneš, Karolína Jankovičová, Dana Vlášková, Ctirad Andrýs
• Format: Article
• Language: English
• Published: Karolinum Press 2009-01-01
• Series: Acta Medica
• Subjects: RANK; RANKL; Monocytes; Cardiac surgery; Cardiopulmonary bypass
• Online Access: https://actamedica.lfhk.cuni.cz/52/4/0149/
• ISSN: 1211-4286; 1805-9694

Background: Cardiac surgery provokes a systemic inflammatory response in any patient. This complex body reaction involves also RANK/RANKL molecules which have been recently identified as principal regulators of bone metabolism. Aims: To follow the changes in the expression of RANK/RANKL molecules on innate immune cells of cardiac surgical patients. Patients and Methods: Twenty-six patients undergoing cardiac surgical were assigned to undergo coronary artery bypass grafting using either cardiopulmonary bypass (“on-pump”) or modified “miniinvasive on-pump”. The expression of RANK/RANKL was performed by flow cytometry. Results: Significantly increased expression of RANK on monocytes of “miniinvasive on-pump” patients was found at the 1st, the 3nd, and 7th postoperative days. The similar pattern was found also for monocyte RANKL expression. In addition, RANKL expression was significantly increased at the 3rd postoperative day in “on-pump” patient. No significant differences between “miniinvasive on-pump” and “on-pump” cardiac surgical patients were found. Conclusion: The expression of both RANK and RANKL molecules is significantly enhanced on monocytes of “miniinvasive on-pump” cardiac surgical patients.