MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCES

MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCES

Random amplified polymorphic DNA (RAPD) analysis was applied for molecular characterization of five Salmonella enteritidis strains from different avian sources and human cases of infection. A total of 16 primers were used and only five primers showed good discriminatory power for all five isolates....

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Main Author: E. YAQOOB, I. HUSSAIN AND S. U. RAHMAN
Format: Article
Language:English
Published: University of Agriculture, Faisalabad 2007-04-01
Series:Pakistan Veterinary Journal
Subjects:
Salmonella enteritidis
RAPD
dendrogram
Online Access:http://pvj.com.pk/pdf-files/27_2/page%20102-104.pdf
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spelling doaj-257a9de276734d50b60bca5b4da8d1b12020-11-24T22:53:19ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182007-04-01272102104MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCESE. YAQOOB, I. HUSSAIN AND S. U. RAHMANRandom amplified polymorphic DNA (RAPD) analysis was applied for molecular characterization of five Salmonella enteritidis strains from different avian sources and human cases of infection. A total of 16 primers were used and only five primers showed good discriminatory power for all five isolates. Dendrogram showed a common lineage among all five isolates. There was a close genetic relationship among isolates of eggs and human sources, while there was less pronounced homology among isolates of broiler meat and human sources. On the basis of results we have found that an endemic strain of S. enteritidis is prevalent between the poultry derived food and humans which gives us an insight to genetic diversity of S. enteritidis from these sources.http://pvj.com.pk/pdf-files/27_2/page%20102-104.pdfSalmonella enteritidisRAPDdendrogram
collection DOAJ
language English
format Article
sources DOAJ
author E. YAQOOB, I. HUSSAIN AND S. U. RAHMAN
spellingShingle E. YAQOOB, I. HUSSAIN AND S. U. RAHMAN
MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCES
Pakistan Veterinary Journal
Salmonella enteritidis
RAPD
dendrogram
author_facet E. YAQOOB, I. HUSSAIN AND S. U. RAHMAN
author_sort E. YAQOOB, I. HUSSAIN AND S. U. RAHMAN
title MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCES
title_short MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCES
title_full MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCES
title_fullStr MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCES
title_full_unstemmed MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCES
title_sort molecular characterization by using random amplified polymorphic dna (rapd) analysis of salmonella enteritidis isolates recovered from avian and human sources
publisher University of Agriculture, Faisalabad
series Pakistan Veterinary Journal
issn 0253-8318
publishDate 2007-04-01
description Random amplified polymorphic DNA (RAPD) analysis was applied for molecular characterization of five Salmonella enteritidis strains from different avian sources and human cases of infection. A total of 16 primers were used and only five primers showed good discriminatory power for all five isolates. Dendrogram showed a common lineage among all five isolates. There was a close genetic relationship among isolates of eggs and human sources, while there was less pronounced homology among isolates of broiler meat and human sources. On the basis of results we have found that an endemic strain of S. enteritidis is prevalent between the poultry derived food and humans which gives us an insight to genetic diversity of S. enteritidis from these sources.
topic Salmonella enteritidis
RAPD
dendrogram
url http://pvj.com.pk/pdf-files/27_2/page%20102-104.pdf
work_keys_str_mv AT eyaqoobihussainandsurahman molecularcharacterizationbyusingrandomamplifiedpolymorphicdnarapdanalysisofsalmonellaenteritidisisolatesrecoveredfromavianandhumansources
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