MOLECULAR CHARACTERIZATION BY USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) ANALYSIS OF SALMONELLA ENTERITIDIS ISOLATES RECOVERED FROM AVIAN AND HUMAN SOURCES
Random amplified polymorphic DNA (RAPD) analysis was applied for molecular characterization of five Salmonella enteritidis strains from different avian sources and human cases of infection. A total of 16 primers were used and only five primers showed good discriminatory power for all five isolates....
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2007-04-01
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| Series: | Pakistan Veterinary Journal |
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| Online Access: | http://pvj.com.pk/pdf-files/27_2/page%20102-104.pdf |
| Summary: | Random amplified polymorphic DNA (RAPD) analysis was applied for molecular characterization of five Salmonella enteritidis strains from different avian sources and human cases of infection. A total of 16 primers were used and only five primers showed good discriminatory power for all five isolates. Dendrogram showed a common lineage among all five isolates. There was a close genetic relationship among isolates of eggs and human sources, while there was less pronounced homology among isolates of broiler meat and human sources. On the basis of results we have found that an endemic strain of S. enteritidis is prevalent between the poultry derived food and humans which gives us an insight to genetic diversity of S. enteritidis from these sources. |
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| ISSN: | 0253-8318 |