| Summary: | ABSTRACT This study was conducted to determine the total lysine requirement for female broiler breeders from days 14 to 42. Two-hundred and ten female broiler breeders were used in a completely randomized design with 6 replicates per treatment and 5 chicks per pen under restricted feeding. The contents of total lysine used in this experiment were 0.68, 0.72, 0.76, 0.80, 0.84, 0.88 and 0.92% in the diet. A basal diet was formulated to meet or to exceed the Ross 308 female broiler breeders’ nutrient specifications except for the lysine. Body weight and feed intake were measured to calculate feed efficiency, and body weight uniformity was defined on a weekly basis. One chick per pen was randomly selected to collect blood samples, organ and an abundance of sexual maturity associated miRNAs (miR-21, mi-26a and mi-375) in the plasma was measured on day 42. Increasing total lysine contents in the diet improved body weight gain and feed conversion ratio from days 14 to 42. The combined values from the two models for BW, average daily gain and feed efficiency were estimated at 1.04, 1.00 and 1.21% total lysine, respectively. Total lysine contents did not affect the ovary weight, serum estradiol-17b, serum progesterone and plasma urea nitrogen or the expression levels of the three miRNAs on day 42. Therefore, the results from the current study indicated that female broiler breeders fed on 0.68% total lysine could achieve the recommended BW, suggested by field practice when data were fitted into the overlapped point of linear-, and quadratic-plateau models.
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