Damage assessment of the equine sperm membranes by fluorimetric technique

Damage assessment of the equine sperm membranes by fluorimetric technique

To validate a practical technique of simultaneous evaluation of the plasma, acrosomal and mitochondrial membranes in equine spermatozoa three fluorescent probes (PI, FITC-PSA and MITO) were associated. Four ejaculates from three stallions (n=12) were diluted in TALP medium and split into 2 aliquots,...

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Bibliographic Details
Main Authors: Eneiva Carla Carvalho Celeghini, André Furugen Cesar de Andrade, Cláudia Fernandes Raphael, Juliana Nascimento, Janahi Souza Ticianelli, Rubens Paes de Arruda
Format: Article
Language:English
Published: Instituto de Tecnologia do Paraná (Tecpar) 2010-12-01
Series:Brazilian Archives of Biology and Technology
Subjects:
Stallion
acrosome
plasma membrane
mitochondria
fluorescent probes
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600004

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