Site-directed mutagenesis of cysteine to serine residues affects heparin binding and mitogenicity in fibroblast growth factor 4 produced in Escherichia coli

Site-directed mutagenesis of cysteine to serine residues affects heparin binding and mitogenicity in fibroblast growth factor 4 produced in Escherichia coli

The binding activity to heparan sulphate is crucial for the mitogenic activity of fibroblast growth factor 4 (FGF4) in developing mammalian embryos. There are two conserved cysteine residues in FGF family proteins, Cys-84 and Cys-151 in mouse FGF4, and these constitute all of the cysteine residues i...

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Bibliographic Details
Main Authors: Yuki Kumagai, Takahiro Kikuchi, Asumi Nonaka, Misuzu Hiraide, Suguru Sato, Mizuki Sakuraoka, Akira Sasaki, Masayuki Kobayashi
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:Biotechnology & Biotechnological Equipment
Subjects:
cell growth
cysteine residues
fgf4
heparin binding
point mutation
recombinant protein
Online Access:http://dx.doi.org/10.1080/13102818.2019.1590161

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