Salivary Immunoglobulin Gamma-3 Chain C Is a Promising Noninvasive Biomarker for Systemic Lupus Erythematosus

• Main Authors: Ju-Yang Jung, Jin-Young Nam, Keun-Sil Ryu, In-Ok Son, Joo-Ho Shin, Wook-Young Baek, Hyoun-Ah Kim, Chang-Hee Suh
• Format: Article
• Language: English
• Published: MDPI AG 2021-01-01
• Series: International Journal of Molecular Sciences
• Subjects: systemic lupus erythematosus; proteomics; salivary proteins and peptides; biomarker
• Online Access: https://www.mdpi.com/1422-0067/22/3/1374

We aimed to characterize the salivary protein components and identify biomarkers in patients with systemic lupus erythematosus (SLE). A proteomic analysis using two-dimensional gel electrophoresis and mass spectrometry was performed to determine the alterations of salivary proteins between patients with SLE and healthy controls, and the concentrations of the candidate proteins were measured through Western blot analysis and the enzyme-linked immunosorbent assay. The 10 differentially expressed protein spots were immunoglobulin gamma-3 chain C region (IGHG3), immunoglobulin alpha-1 chain C region, protein S100A8, lactoferrin, leukemia-associated protein 7, and 8-oxoguanine DNA glycosylase. The patients with SLE exhibited enhanced salivary IGHG3 (3.9 ± 2.15 pg/mL) and lactoferrin (4.7 ± 1.8 pg/mL) levels compared to patients with rheumatoid arthritis (1.8 ± 1.01 pg/mL and 3.2 ± 1.6 pg/mL, respectively; <i>p</i> < 0.001 for both) or healthy controls (2.2 ± 1.64 pg/mL and 2.2 ± 1.7 pg/mL, respectively; <i>p</i> < 0.001 for both). The salivary IGHG3 levels correlated with the erythrocyte sedimentation rate (<i>r</i> = 0.26, <i>p</i> = 0.01), anti-double-stranded DNA (dsDNA) antibody levels (<i>r</i> = 0.25, <i>p</i> = 0.01), and nephritis (<i>r</i> = 0.28, <i>p</i> = 0.01). The proteomic analysis revealed that the salivary IGHG3 levels were associated with SLE and lupus disease activity, suggesting that salivary IGHG3 may be a promising noninvasive biomarker for SLE.