Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach

Being the second cause of mortality across the globe, there is now a persistent effort to establish new cancer medication and therapies. Any accomplishment in treating cancers entails the existence of accurate identification systems empowering the early diagnosis. Recent studies indicate CTCs&rs...

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Main Authors: Amir Shamloo, Saba Ahmad, Maede Momeni
Format: Article
Language:English
Published: MDPI AG 2018-06-01
Series:Biosensors
Subjects:
Online Access:http://www.mdpi.com/2079-6374/8/2/56
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spelling doaj-2525b572c6f945dbb6469e6eec3065692020-11-24T23:35:33ZengMDPI AGBiosensors2079-63742018-06-01825610.3390/bios8020056bios8020056Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical ApproachAmir Shamloo0Saba Ahmad1Maede Momeni2School of Mechanical Engineering, Sharif University of Technology, Azadi Ave., 11155-9567 Tehran, IranSchool of Mechanical Engineering, Sharif University of Technology, Azadi Ave., 11155-9567 Tehran, IranSchool of Mechanical Engineering, Sharif University of Technology, Azadi Ave., 11155-9567 Tehran, IranBeing the second cause of mortality across the globe, there is now a persistent effort to establish new cancer medication and therapies. Any accomplishment in treating cancers entails the existence of accurate identification systems empowering the early diagnosis. Recent studies indicate CTCs’ potential in cancer prognosis as well as therapy monitoring. The chief shortcoming with CTCs is that they are exceedingly rare cells in their clinically relevant concentration. Here, we simulated a microfluidic construct devised for immunomagnetic separation of the particles of interest from the background cells. This separation unit is integrated with a mixer subunit. The mixer is envisioned for mixing the CTC enriched stream with lysis buffer to extract the biological material of the cell. Some modification was proposed on mixing geometry improving the efficacy of the functional unit. A valuation of engaged forces was made and some forces were neglected due to their order of magnitude. The position of the magnet was also optimized by doing parametric study. For the mixer unit, the effect of applied voltage and frequency on mixing index was studied to find the optimal voltage and frequency which provides better mixing. Above-mentioned studies were done on isolated units and the effect of each functional unit on the other is not studied. As the final step, an integrated microfluidic platform composed of both functional subunits was simulated simultaneously. To ensure the independence of results from the grid, grid studies were also performed. The studies carried out on the construct reveal its potential for diagnostic application.http://www.mdpi.com/2079-6374/8/2/56microfluidic biochipcirculating tumor cellmagnetophoretic separationelectroosmotic mixingnumerical simulationgeometry optimization
collection DOAJ
language English
format Article
sources DOAJ
author Amir Shamloo
Saba Ahmad
Maede Momeni
spellingShingle Amir Shamloo
Saba Ahmad
Maede Momeni
Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach
Biosensors
microfluidic biochip
circulating tumor cell
magnetophoretic separation
electroosmotic mixing
numerical simulation
geometry optimization
author_facet Amir Shamloo
Saba Ahmad
Maede Momeni
author_sort Amir Shamloo
title Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach
title_short Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach
title_full Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach
title_fullStr Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach
title_full_unstemmed Design and Parameter Study of Integrated Microfluidic Platform for CTC Isolation and Enquiry; A Numerical Approach
title_sort design and parameter study of integrated microfluidic platform for ctc isolation and enquiry; a numerical approach
publisher MDPI AG
series Biosensors
issn 2079-6374
publishDate 2018-06-01
description Being the second cause of mortality across the globe, there is now a persistent effort to establish new cancer medication and therapies. Any accomplishment in treating cancers entails the existence of accurate identification systems empowering the early diagnosis. Recent studies indicate CTCs’ potential in cancer prognosis as well as therapy monitoring. The chief shortcoming with CTCs is that they are exceedingly rare cells in their clinically relevant concentration. Here, we simulated a microfluidic construct devised for immunomagnetic separation of the particles of interest from the background cells. This separation unit is integrated with a mixer subunit. The mixer is envisioned for mixing the CTC enriched stream with lysis buffer to extract the biological material of the cell. Some modification was proposed on mixing geometry improving the efficacy of the functional unit. A valuation of engaged forces was made and some forces were neglected due to their order of magnitude. The position of the magnet was also optimized by doing parametric study. For the mixer unit, the effect of applied voltage and frequency on mixing index was studied to find the optimal voltage and frequency which provides better mixing. Above-mentioned studies were done on isolated units and the effect of each functional unit on the other is not studied. As the final step, an integrated microfluidic platform composed of both functional subunits was simulated simultaneously. To ensure the independence of results from the grid, grid studies were also performed. The studies carried out on the construct reveal its potential for diagnostic application.
topic microfluidic biochip
circulating tumor cell
magnetophoretic separation
electroosmotic mixing
numerical simulation
geometry optimization
url http://www.mdpi.com/2079-6374/8/2/56
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