NRP-1 gene expression in nasopharyngeal carcinoma tissue and regulating effect of targeted inhibition of gene expression on cell function
Objective: To study NRP-1 gene expression in nasopharyngeal carcinoma tissue and the regulating effect of targeted inhibition of gene expression on cell function. Methods: Nasopharyngeal carcinoma tissue, adjacent tissue and normal tissue were collected, and NRP-1 expression levels were detected;...
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Board of Journal of Hainan Medical University
2016-07-01
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| Series: | Journal of Hainan Medical University |
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| Online Access: | http://www.hnykdxxb.com/PDF/201613/2.pdf |
| Summary: | Objective: To study NRP-1 gene expression in nasopharyngeal carcinoma tissue and
the regulating effect of targeted inhibition of gene expression on cell function. Methods:
Nasopharyngeal carcinoma tissue, adjacent tissue and normal tissue were collected, and
NRP-1 expression levels were detected; nasopharyngeal carcinoma CNE-2 cells were cultured
and transfected with NRP-1 siRNA and negative control (NC) siRNA, and the contents of
angiogenesis molecules, proliferation-related proteins and invasion-related proteins were
detected. Results: NRP-1 protein content, positive cell rate, staining intensity and IHC score in
nasopharyngeal carcinoma tissue were higher than those in adjacent tissue and normal tissue;
VEGF, bFGF, EGF and ANG-1 contents in culture medium of NRP1-siRNA group were lower
than those in NC-siRNA group; Tie-2, EGFR, Livin, Bmi-1, CDK6, CDK4, CCND1, E2F1,
LMP1, FN1, MT1-MMP and Fascin expression levels in cells of NRP1-siRNA group were
lower than those in NC-siRNA group. Conclusions: NRP-1 expression level significantly
increases in nasopharyngeal carcinoma tissue, and NRP-1 can promote nasopharyngeal
carcinoma angiogenesis, cell proliferation and invasion. |
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| ISSN: | 1007-1237 1007-1237 |