Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo

Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo

The recent large outbreaks of Ebola virus disease (EVD) in West Africa and the Democratic Republic of the Congo (DRC) have highlighted the need for rapid diagnostic tests to control this disease. In this study, we clinically evaluated a previously developed immunochromatography-based kit, QuickNavi&...

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Main Authors: Sheila Makiala, Daniel Mukadi, Anja De Weggheleire, Shino Muramatsu, Daisuke Kato, Koichi Inano, Fumio Gondaira, Masahiro Kajihara, Reiko Yoshida, Katendi Changula, Aaron Mweene, Placide Mbala-Kingebeni, Jean-Jacques Muyembe-Tamfum, Justin Masumu, Steve Ahuka, Ayato Takada
Format: Article
Language:English
Published: MDPI AG 2019-06-01
Series:Viruses
Subjects:
Ebola virus
EVD
rapid diagnostic test
immunochromatography
QuickNavi
DRC
Online Access:https://www.mdpi.com/1999-4915/11/7/589
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spelling doaj-25123d26cf004dca9f5c11fd454e62472020-11-25T01:02:27ZengMDPI AGViruses1999-49152019-06-0111758910.3390/v11070589v11070589Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the CongoSheila Makiala0Daniel Mukadi1Anja De Weggheleire2Shino Muramatsu3Daisuke Kato4Koichi Inano5Fumio Gondaira6Masahiro Kajihara7Reiko Yoshida8Katendi Changula9Aaron Mweene10Placide Mbala-Kingebeni11Jean-Jacques Muyembe-Tamfum12Justin Masumu13Steve Ahuka14Ayato Takada15Institut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the CongoInstitut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the CongoDepartment of Clinical Sciences, Institute of Tropical Medicine, Antwerp, BelgiumDenka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, JapanDenka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, JapanDenka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, JapanDenka Seiken Co., Ltd., 1359-1, Kagamida, Kigoshi, Gosen, Niigata 959-1695, JapanDivision of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, JapanDivision of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, JapanSchool of Veterinary Medicine, the University of Zambia, Great East Road Campus, Lusaka, ZambiaSchool of Veterinary Medicine, the University of Zambia, Great East Road Campus, Lusaka, ZambiaInstitut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the CongoInstitut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the CongoInstitut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the CongoInstitut National de Recherche Biomédicale, Avenue de la Démocratie, Kinshasa/Gombe-P.O. Box 1197, Kinshasa I, Democratic Republic of the CongoDivision of Global Epidemiology, Research Center for Zoonosis Control, Hokkaido University, Kita-20, Nishi-10, Kita-ku, Sapporo 001-0020, JapanThe recent large outbreaks of Ebola virus disease (EVD) in West Africa and the Democratic Republic of the Congo (DRC) have highlighted the need for rapid diagnostic tests to control this disease. In this study, we clinically evaluated a previously developed immunochromatography-based kit, QuickNavi<sup>TM</sup>-Ebola. During the 2018 outbreaks in DRC, 928 blood samples from EVD-suspected cases were tested with QuickNavi<sup>TM</sup>-Ebola and the WHO-approved GeneXpert. The sensitivity and specificity of QuickNavi<sup>TM</sup>-Ebola, estimated by comparing it to GeneXpert-confirmed cases, were 85% (68/80) and 99.8% (846/848), respectively. These results indicate the practical reliability of QuickNavi<sup>TM</sup>-Ebola for point-of-care diagnosis of EVD.https://www.mdpi.com/1999-4915/11/7/589Ebola virusEVDrapid diagnostic testimmunochromatographyQuickNaviDRC
collection DOAJ
language English
format Article
sources DOAJ
author Sheila Makiala
Daniel Mukadi
Anja De Weggheleire
Shino Muramatsu
Daisuke Kato
Koichi Inano
Fumio Gondaira
Masahiro Kajihara
Reiko Yoshida
Katendi Changula
Aaron Mweene
Placide Mbala-Kingebeni
Jean-Jacques Muyembe-Tamfum
Justin Masumu
Steve Ahuka
Ayato Takada
spellingShingle Sheila Makiala
Daniel Mukadi
Anja De Weggheleire
Shino Muramatsu
Daisuke Kato
Koichi Inano
Fumio Gondaira
Masahiro Kajihara
Reiko Yoshida
Katendi Changula
Aaron Mweene
Placide Mbala-Kingebeni
Jean-Jacques Muyembe-Tamfum
Justin Masumu
Steve Ahuka
Ayato Takada
Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo
Viruses
Ebola virus
EVD
rapid diagnostic test
immunochromatography
QuickNavi
DRC
author_facet Sheila Makiala
Daniel Mukadi
Anja De Weggheleire
Shino Muramatsu
Daisuke Kato
Koichi Inano
Fumio Gondaira
Masahiro Kajihara
Reiko Yoshida
Katendi Changula
Aaron Mweene
Placide Mbala-Kingebeni
Jean-Jacques Muyembe-Tamfum
Justin Masumu
Steve Ahuka
Ayato Takada
author_sort Sheila Makiala
title Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo
title_short Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo
title_full Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo
title_fullStr Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo
title_full_unstemmed Clinical Evaluation of QuickNavi<sup>TM</sup>-Ebola in the 2018 Outbreak of Ebola Virus Disease in the Democratic Republic of the Congo
title_sort clinical evaluation of quicknavi<sup>tm</sup>-ebola in the 2018 outbreak of ebola virus disease in the democratic republic of the congo
publisher MDPI AG
series Viruses
issn 1999-4915
publishDate 2019-06-01
description The recent large outbreaks of Ebola virus disease (EVD) in West Africa and the Democratic Republic of the Congo (DRC) have highlighted the need for rapid diagnostic tests to control this disease. In this study, we clinically evaluated a previously developed immunochromatography-based kit, QuickNavi<sup>TM</sup>-Ebola. During the 2018 outbreaks in DRC, 928 blood samples from EVD-suspected cases were tested with QuickNavi<sup>TM</sup>-Ebola and the WHO-approved GeneXpert. The sensitivity and specificity of QuickNavi<sup>TM</sup>-Ebola, estimated by comparing it to GeneXpert-confirmed cases, were 85% (68/80) and 99.8% (846/848), respectively. These results indicate the practical reliability of QuickNavi<sup>TM</sup>-Ebola for point-of-care diagnosis of EVD.
topic Ebola virus
EVD
rapid diagnostic test
immunochromatography
QuickNavi
DRC
url https://www.mdpi.com/1999-4915/11/7/589
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