Cysteine Proteinase-1 and Cut Protein Isoform Control Dendritic Innervation of Two Distinct Sensory Fields by a Single Neuron
Dendrites often exhibit structural changes in response to local inputs. Although mechanisms that pattern and maintain dendritic arbors are becoming clearer, processes regulating regrowth, during context-dependent plasticity or after injury, remain poorly understood. We found that a class of Drosophi...
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2014-03-01
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doaj-25046ed14972416cbbf18d3ef341e9212020-11-24T21:47:27ZengElsevierCell Reports2211-12472014-03-016578379110.1016/j.celrep.2014.02.003Cysteine Proteinase-1 and Cut Protein Isoform Control Dendritic Innervation of Two Distinct Sensory Fields by a Single NeuronGray R. Lyons0Ryan O. Andersen1Khadar Abdi2Won-Seok Song3Chay T. Kuo4Department of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USADepartment of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USADepartment of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USADepartment of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USADepartment of Cell Biology, Duke University School of Medicine, Durham, NC 27710, USADendrites often exhibit structural changes in response to local inputs. Although mechanisms that pattern and maintain dendritic arbors are becoming clearer, processes regulating regrowth, during context-dependent plasticity or after injury, remain poorly understood. We found that a class of Drosophila sensory neurons, through complete pruning and regeneration, can elaborate two distinct dendritic trees, innervating independent sensory fields. An expression screen identified Cysteine proteinase-1 (Cp1) as a critical regulator of this process. Unlike known ecdysone effectors, Cp1-mutant ddaC neurons pruned larval dendrites normally but failed to regrow adult dendrites. Cp1 expression was upregulated/concentrated in the nucleus during metamorphosis, controlling production of a truncated Cut homeodomain transcription factor. This truncated Cut, but not the full-length protein, allowed Cp1-mutant ddaC neurons to regenerate higher-order adult dendrites. These results identify a molecular pathway needed for dendrite regrowth after pruning, which allows the same neuron to innervate distinct sensory fields.http://www.sciencedirect.com/science/article/pii/S2211124714000825 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Gray R. Lyons Ryan O. Andersen Khadar Abdi Won-Seok Song Chay T. Kuo |
spellingShingle |
Gray R. Lyons Ryan O. Andersen Khadar Abdi Won-Seok Song Chay T. Kuo Cysteine Proteinase-1 and Cut Protein Isoform Control Dendritic Innervation of Two Distinct Sensory Fields by a Single Neuron Cell Reports |
author_facet |
Gray R. Lyons Ryan O. Andersen Khadar Abdi Won-Seok Song Chay T. Kuo |
author_sort |
Gray R. Lyons |
title |
Cysteine Proteinase-1 and Cut Protein Isoform Control Dendritic Innervation of Two Distinct Sensory Fields by a Single Neuron |
title_short |
Cysteine Proteinase-1 and Cut Protein Isoform Control Dendritic Innervation of Two Distinct Sensory Fields by a Single Neuron |
title_full |
Cysteine Proteinase-1 and Cut Protein Isoform Control Dendritic Innervation of Two Distinct Sensory Fields by a Single Neuron |
title_fullStr |
Cysteine Proteinase-1 and Cut Protein Isoform Control Dendritic Innervation of Two Distinct Sensory Fields by a Single Neuron |
title_full_unstemmed |
Cysteine Proteinase-1 and Cut Protein Isoform Control Dendritic Innervation of Two Distinct Sensory Fields by a Single Neuron |
title_sort |
cysteine proteinase-1 and cut protein isoform control dendritic innervation of two distinct sensory fields by a single neuron |
publisher |
Elsevier |
series |
Cell Reports |
issn |
2211-1247 |
publishDate |
2014-03-01 |
description |
Dendrites often exhibit structural changes in response to local inputs. Although mechanisms that pattern and maintain dendritic arbors are becoming clearer, processes regulating regrowth, during context-dependent plasticity or after injury, remain poorly understood. We found that a class of Drosophila sensory neurons, through complete pruning and regeneration, can elaborate two distinct dendritic trees, innervating independent sensory fields. An expression screen identified Cysteine proteinase-1 (Cp1) as a critical regulator of this process. Unlike known ecdysone effectors, Cp1-mutant ddaC neurons pruned larval dendrites normally but failed to regrow adult dendrites. Cp1 expression was upregulated/concentrated in the nucleus during metamorphosis, controlling production of a truncated Cut homeodomain transcription factor. This truncated Cut, but not the full-length protein, allowed Cp1-mutant ddaC neurons to regenerate higher-order adult dendrites. These results identify a molecular pathway needed for dendrite regrowth after pruning, which allows the same neuron to innervate distinct sensory fields. |
url |
http://www.sciencedirect.com/science/article/pii/S2211124714000825 |
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