The IL-6 -634C/G polymorphism: a candidate genetic marker for the prediction of idiopathic recurrent pregnancy loss

Background: Recurrent pregnancy loss (RPL) is defined as two or more miscarriages before the 20th week of gestation and its etiology is unknown in 50% of the cases. Interleukin 6 is an immune mediator, plays a regulatory role in embryo implantation and placental development. Objective: The purpose...

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Main Authors: Zarnegar Rasti, Mahboobeh Nasiri, Leila Kohan
Format: Article
Language:English
Published: Shahid Sadoughi University of Medical Science, Yazd, Iran 2016-02-01
Series:Iranian Journal of Reproductive Medicine
Online Access:http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/1827/950
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spelling doaj-250122ee6c754c6d865b83c5703951ad2020-11-24T21:55:38ZengShahid Sadoughi University of Medical Science, Yazd, IranIranian Journal of Reproductive Medicine1680-64332008-21772016-02-01142103108The IL-6 -634C/G polymorphism: a candidate genetic marker for the prediction of idiopathic recurrent pregnancy lossZarnegar Rasti0Mahboobeh Nasiri1Leila Kohan2Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, Iran.Department of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, IranDepartment of Biology, Islamic Azad University, Arsanjan Branch, Arsanjan, IranBackground: Recurrent pregnancy loss (RPL) is defined as two or more miscarriages before the 20th week of gestation and its etiology is unknown in 50% of the cases. Interleukin 6 is an immune mediator, plays a regulatory role in embryo implantation and placental development. Objective: The purpose was to assess the association between IL-6 -634C/G polymorphism and, susceptibility to idiopathic RPL for the first time in Iran. Materials and Methods: In total 121 women with RPL and 121 healthy women as control group were enrolled in this case-control study. This study was performed from August 2013 to October 2014 in the Molecular Genetics Laboratory of Arsanjan University. Candidate polymorphism was evaluated by PCR-RFLP method on extracted genomic DNA. Data was analyzed using the statistical SPSS package. Results: Our results showed an increased risk of RPL in patients with GG + GC genotype (OR=5.1, 95%CI: 1.04-25.3, p=0.04) in comparison to CC genotype. The frequency of mutant allele G in patients and controls was 0.75 and 0.66 respectively. The mutant allele G predisposes women to miscarriage 1.5 times greater than controls (OR=1.5, 95%CI: 1.03-2.27, p=0.036). The mean number of live births in RPL women (1.3±2.3) was significantly lower compared to control women (4.8±2.3). Conclusion: This study indicated that the promoter polymorphism (-634C/G) of the IL-6 gene has likely influence on individual susceptibility to RPL.http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/1827/950
collection DOAJ
language English
format Article
sources DOAJ
author Zarnegar Rasti
Mahboobeh Nasiri
Leila Kohan
spellingShingle Zarnegar Rasti
Mahboobeh Nasiri
Leila Kohan
The IL-6 -634C/G polymorphism: a candidate genetic marker for the prediction of idiopathic recurrent pregnancy loss
Iranian Journal of Reproductive Medicine
author_facet Zarnegar Rasti
Mahboobeh Nasiri
Leila Kohan
author_sort Zarnegar Rasti
title The IL-6 -634C/G polymorphism: a candidate genetic marker for the prediction of idiopathic recurrent pregnancy loss
title_short The IL-6 -634C/G polymorphism: a candidate genetic marker for the prediction of idiopathic recurrent pregnancy loss
title_full The IL-6 -634C/G polymorphism: a candidate genetic marker for the prediction of idiopathic recurrent pregnancy loss
title_fullStr The IL-6 -634C/G polymorphism: a candidate genetic marker for the prediction of idiopathic recurrent pregnancy loss
title_full_unstemmed The IL-6 -634C/G polymorphism: a candidate genetic marker for the prediction of idiopathic recurrent pregnancy loss
title_sort il-6 -634c/g polymorphism: a candidate genetic marker for the prediction of idiopathic recurrent pregnancy loss
publisher Shahid Sadoughi University of Medical Science, Yazd, Iran
series Iranian Journal of Reproductive Medicine
issn 1680-6433
2008-2177
publishDate 2016-02-01
description Background: Recurrent pregnancy loss (RPL) is defined as two or more miscarriages before the 20th week of gestation and its etiology is unknown in 50% of the cases. Interleukin 6 is an immune mediator, plays a regulatory role in embryo implantation and placental development. Objective: The purpose was to assess the association between IL-6 -634C/G polymorphism and, susceptibility to idiopathic RPL for the first time in Iran. Materials and Methods: In total 121 women with RPL and 121 healthy women as control group were enrolled in this case-control study. This study was performed from August 2013 to October 2014 in the Molecular Genetics Laboratory of Arsanjan University. Candidate polymorphism was evaluated by PCR-RFLP method on extracted genomic DNA. Data was analyzed using the statistical SPSS package. Results: Our results showed an increased risk of RPL in patients with GG + GC genotype (OR=5.1, 95%CI: 1.04-25.3, p=0.04) in comparison to CC genotype. The frequency of mutant allele G in patients and controls was 0.75 and 0.66 respectively. The mutant allele G predisposes women to miscarriage 1.5 times greater than controls (OR=1.5, 95%CI: 1.03-2.27, p=0.036). The mean number of live births in RPL women (1.3±2.3) was significantly lower compared to control women (4.8±2.3). Conclusion: This study indicated that the promoter polymorphism (-634C/G) of the IL-6 gene has likely influence on individual susceptibility to RPL.
url http://www.ssu.ac.ir/ijrm/index.php/ijrm/article/view/1827/950
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