Bovine viral diarrhea diagnostic: immunohistochemistry standardization for routine Padronização da técnica de imunoistoquímica para o diagnóstico etiológico de rotina da diarréia bovina a vírus

• Main Authors: G.I. Andrade, C.V. Serra, E.F. Barbosa-Stancioli, R. Serakides, Z.I.P. Lobato
• Format: Article
• Language: English
• Published: Universidade Federal de Minas Gerais 2005-10-01
• Series: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
• Subjects: bovino; diarréia bovina a vírus; imunoistoquímica; bovine viral diarrhea; virus; immunohistochemistry
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000500018

The immunohistochemistry standardization for bovine viral diarrhea virus (BVDV) diagnostic was described. The formalin-fixed tissue samples from a heifer with mucosal disease were used as positive control. The validation of the first phase results was performed using samples from an aborted fetus and a calf infected with reference strains of BVDV. The best results were seen using monoclonal antibodies and a commercial kit consisting of labelled streptavidin biotin (LSAB) reagents and the diaminobenzidine (DAB) substrate-chromogen reagent. The immunohistochemistry demonstrated to be an useful method for routine diagnosis for the controll and detection of BVDV infection.