| Summary: | <p>Abstract</p> <p>Background</p> <p>Efflux pumps belonging to the resistance-nodulation-division (RND) superfamily in bacteria are involved in antibiotic resistance and solvent tolerance but have an unknown physiological role. EmhABC, a RND-type efflux pump in <it>Pseudomonas fluorescens </it>strain cLP6a, extrudes hydrophobic antibiotics, dyes and polycyclic aromatic hydrocarbons including phenanthrene. The effects of physico-chemical factors such as temperature or antibiotics on the activity and expression of EmhABC were determined in order to deduce its physiological role(s) in strain cLP6a in comparison to the <it>emhB </it>disruptant strain, cLP6a-1.</p> <p>Results</p> <p>Efflux assays conducted with <sup>14</sup>C-phenanthrene showed that EmhABC activity is affected by incubation temperature. Increased phenanthrene efflux was measured in cLP6a cells grown at 10°C and decreased efflux was observed at 35°C compared with cells grown at the optimum temperature of 28°C. Membrane fatty acids in cLP6a cells were substantially altered by changes in growth temperature and in the presence of tetracycline. Changed membrane fatty acids and increased membrane permeability were associated with ~30-fold increased expression <it>of emhABC </it>in cLP6a cells grown at 35°C, and with increased extracellular free fatty acids. Growth <it>of P. fluorescens </it>cLP6a at supra-optimal temperature was enhanced by the presence of EmhABC compared to strain cLP6a-1.</p> <p>Conclusions</p> <p>Combined, these observations suggest that the EmhABC efflux pump may be involved in the management of membrane stress effects such as those due to unfavourable incubation temperatures. Efflux of fatty acids replaced as a result of membrane damage or phospholipid turnover may be the primary physiological role of the EmhABC efflux pump in <it>P. fluorescens </it>cLP6a.</p>
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