Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells

Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells

Erythropoietin (EPO) exerts (renal) tissue protective effects. Since it is unclear whether this is a direct effect of EPO on the kidney or not, we investigated whether EPO is able to protect human renal tubular epithelial cells (hTECs) from oxidative stress and if so which pathways are involved. EPO...

Full description

Bibliographic Details
Main Authors: Annelies De Beuf, Xiang-hua Hou, Patrick C. D'Haese, Anja Verhulst
Format: Article
Language:English
Published: Hindawi Limited 2010-01-01
Series:Journal of Biomedicine and Biotechnology
Online Access:http://dx.doi.org/10.1155/2010/395785
id doaj-24a0837b7c3c4dd79997fafa9f73233d
record_format Article
spelling doaj-24a0837b7c3c4dd79997fafa9f73233d2020-11-25T00:53:35ZengHindawi LimitedJournal of Biomedicine and Biotechnology1110-72431110-72512010-01-01201010.1155/2010/395785395785Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular CellsAnnelies De Beuf0Xiang-hua Hou1Patrick C. D'Haese2Anja Verhulst3Laboratory of Pathophysiology, Faculties of Medicine and Biomedical, Pharmaceutical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, BelgiumLaboratory of Pathophysiology, Faculties of Medicine and Biomedical, Pharmaceutical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, BelgiumLaboratory of Pathophysiology, Faculties of Medicine and Biomedical, Pharmaceutical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, BelgiumLaboratory of Pathophysiology, Faculties of Medicine and Biomedical, Pharmaceutical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, 2610 Antwerp, BelgiumErythropoietin (EPO) exerts (renal) tissue protective effects. Since it is unclear whether this is a direct effect of EPO on the kidney or not, we investigated whether EPO is able to protect human renal tubular epithelial cells (hTECs) from oxidative stress and if so which pathways are involved. EPO (epoetin delta) could protect hTECs against oxidative stress by a dose-dependent inhibition of reactive oxygen species formation. This protective effect is possibly related to the membranous expression of the EPO receptor (EPOR) since our data point to the membranous EPOR expression as a prerequisite for this protective effect. Oxidative stress reduction went along with the upregulation of renoprotective genes. Whilst three of these, heme oxygenase-1 (HO-1), aquaporin-1 (AQP-1), and B-cell CLL/lymphoma 2 (Bcl-2) have already been associated with EPO-induced renoprotection, this study for the first time suggests carboxypeptidase M (CPM), dipeptidyl peptidase IV (DPPIV), and cytoglobin (Cygb) to play a role in this process.http://dx.doi.org/10.1155/2010/395785
collection DOAJ
language English
format Article
sources DOAJ
author Annelies De Beuf
Xiang-hua Hou
Patrick C. D'Haese
Anja Verhulst
spellingShingle Annelies De Beuf
Xiang-hua Hou
Patrick C. D'Haese
Anja Verhulst
Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells
Journal of Biomedicine and Biotechnology
author_facet Annelies De Beuf
Xiang-hua Hou
Patrick C. D'Haese
Anja Verhulst
author_sort Annelies De Beuf
title Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells
title_short Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells
title_full Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells
title_fullStr Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells
title_full_unstemmed Epoetin Delta Reduces Oxidative Stress in Primary Human Renal Tubular Cells
title_sort epoetin delta reduces oxidative stress in primary human renal tubular cells
publisher Hindawi Limited
series Journal of Biomedicine and Biotechnology
issn 1110-7243
1110-7251
publishDate 2010-01-01
description Erythropoietin (EPO) exerts (renal) tissue protective effects. Since it is unclear whether this is a direct effect of EPO on the kidney or not, we investigated whether EPO is able to protect human renal tubular epithelial cells (hTECs) from oxidative stress and if so which pathways are involved. EPO (epoetin delta) could protect hTECs against oxidative stress by a dose-dependent inhibition of reactive oxygen species formation. This protective effect is possibly related to the membranous expression of the EPO receptor (EPOR) since our data point to the membranous EPOR expression as a prerequisite for this protective effect. Oxidative stress reduction went along with the upregulation of renoprotective genes. Whilst three of these, heme oxygenase-1 (HO-1), aquaporin-1 (AQP-1), and B-cell CLL/lymphoma 2 (Bcl-2) have already been associated with EPO-induced renoprotection, this study for the first time suggests carboxypeptidase M (CPM), dipeptidyl peptidase IV (DPPIV), and cytoglobin (Cygb) to play a role in this process.
url http://dx.doi.org/10.1155/2010/395785
work_keys_str_mv AT anneliesdebeuf epoetindeltareducesoxidativestressinprimaryhumanrenaltubularcells
AT xianghuahou epoetindeltareducesoxidativestressinprimaryhumanrenaltubularcells
AT patrickcdhaese epoetindeltareducesoxidativestressinprimaryhumanrenaltubularcells
AT anjaverhulst epoetindeltareducesoxidativestressinprimaryhumanrenaltubularcells
_version_ 1725237521665753088

Similar Items