First report on the association of a begomovirus with chrysanthemum indicum exhibiting yellowing of leaf vein disease characterized by molecular studies

• Main Authors: Marwal Avinash, Sahu Anurag Kumar, Gaur Rajarshi Kumar
• Format: Article
• Language: English
• Published: Sciendo 2013-12-01
• Series: Journal of Horticultural Research
• Subjects: chrysanthemum indicum plants; begomovirus; pcr; sequencing; southern blot
• Online Access: https://doi.org/10.2478/johr-2013-0017
• ISSN: 2300-5009

Infected leaf samples of an ornamental plant Chrysanthemum indicum showing yellowing of leaf veins were collected from gardens of New Delhi (India). An expected PCR product of size ~500 bp was amplified from total DNA extracts of symptomatic leaf samples with universal primers on the gene of coat protein region of begomovirus DNA-A component. The presence of begomoviruses was also confirmed by Southern blot analysis using control cloned DNA-A probe of Cotton leaf curl virus. Sequence analysis of the virus infecting Chrysanthemum indicum showed 99% nucleotide sequence identity with Clerodendron yellow mosaic virus (EF408037).