RNA <i>In Situ</i> Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of <i>Drosophila</i> Species

RNA <i>In Situ</i> Hybridization for Detecting Gene Expression Patterns in the Abdomens and Wings of <i>Drosophila</i> Species

RNA <i>in situ</i> hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model <i>Drosophila</i> sp...

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Bibliographic Details
Main Authors: Mujeeb Shittu, Tessa Steenwinkel, William Dion, Nathan Ostlund, Komal Raja, Thomas Werner
Format: Article
Language:English
Published: MDPI AG 2021-03-01
Series:Methods and Protocols
Subjects:
<i>Drosophila</i>
<i>in situ</i> hybridization
gene expression
Online Access:https://www.mdpi.com/2409-9279/4/1/20
Description
Summary:RNA <i>in situ</i> hybridization (ISH) is used to visualize spatio-temporal gene expression patterns with broad applications in biology and biomedicine. Here we provide a protocol for mRNA ISH in developing pupal wings and abdomens for model and non-model <i>Drosophila</i> species. We describe best practices in pupal staging, tissue preparation, probe design and synthesis, imaging of gene expression patterns, and image-editing techniques. This protocol has been successfully used to investigate the roles of genes underlying the evolution of novel color patterns in non-model <i>Drosophila</i> species.
ISSN:2409-9279

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