| Summary: | <p>Calli were initiated from corms of <em>Gladiolus hybrida</em> variety Princess Lee by culturing on Murashige and Skoog (MS) medium supplemented with 1.0 mg/l napthaline acetic acid (NAA) and 20 g/l sucrose<strong>. </strong><em>In vitro</em> shoots were regenerated from the calli by transferring into MS medium containing 30 g/l sucrose without growth regulators. Regenerated shoots produced cormlets at the base of the shoot on full or half strength MS medium supplemented with 40-60 g/l sucrose. Highest number of large corms (diameter>1.5 cm) was obtained in the half strength MS medium containing 60 g/l sucrose. Multiplication of <em>in vitro</em>-grown cormlets was achieved by transferring them on to half strength MS medium containing 60 g/l sucrose with 5 or 10 mg/l gibberellic acid (GA<sub>3</sub>). Multiplied corms were successfully acclimatized on a medium containing soil: sand: brick pieces at 1:1:1 ratio. Acclimatized cormlets were established on two different potting mixtures i.e. sand: leaf mould: brick pieces (1:1:1) or sand: compost: brick pieces (1:1:1) after pre-treating with or without 100 mg/l GA<sub>3</sub> for 24 hours. Early sprouting (13 days) was observed in cormlets treated with 100 mg/l GA<sub>3</sub> compared to cormlets without any GA<sub>3</sub> treatment (28 days) and, the highest sprouting percentage (83.3 %) was observed on potting mixture containing sand, leaf mould and brick pieces (1:1:1) under greenhouse conditions.</p><p><em>Tropical Agricultural Research Vol. 23 (1): 1–10 (2011)</em></p><a href="/index.php/TAR/editor/viewMetadata/DOI: http:/dx.doi.org/10.4038/tar.v23i1.4626"> DOI: http://dx.doi.org/<span>10.4038/tar.v23i1.4626</span></a>
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