Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae
Macroautophagy, a major degradation pathway of cytoplasmic components, is carried out through formation of a double-membrane structure, the autophagosome. Although the involvement of specific lipid species in the formation process remains largely obscure, we recently showed that mono-unsaturated fat...
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doaj-243e5b1e1fa243c78fcb64146d89df902021-06-02T19:22:37ZengThe Company of BiologistsBiology Open2046-63902017-01-0161354010.1242/bio.022053022053Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiaeYuta Ogasawara0Shintaro Kira1Yukio Mukai2Takeshi Noda3Akitsugu Yamamoto4 Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, Shiga 526-0829, Japan Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, Shiga 526-0829, Japan Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, Shiga 526-0829, Japan Macroautophagy, a major degradation pathway of cytoplasmic components, is carried out through formation of a double-membrane structure, the autophagosome. Although the involvement of specific lipid species in the formation process remains largely obscure, we recently showed that mono-unsaturated fatty acids (MUFA) generated by stearoyl-CoA desaturase 1 (SCD1) are required for autophagosome formation in mammalian cells. To obtain further insight into the role of MUFA in autophagy, in this study we analyzed the autophagic phenotypes of the yeast mutant of OLE1, an orthologue of SCD1. Δole1 cells were defective in nitrogen starvation-induced autophagy, and the Cvt pathway, when oleic acid was not supplied. Defects in elongation of the isolation membrane led to a defect in autophagosome formation. In the absence of Ole1, the transmembrane protein Atg9 was not able to reach the pre-autophagosomal structure (PAS), the site of autophagosome formation. Thus, autophagosome formation requires Ole1 during the delivery of Atg9 to the PAS/autophagosome from its cellular reservoir.http://bio.biologists.org/content/6/1/35AutophagyUnsaturated fatty acidOLE1Fatty acid monodesaturation |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuta Ogasawara Shintaro Kira Yukio Mukai Takeshi Noda Akitsugu Yamamoto |
spellingShingle |
Yuta Ogasawara Shintaro Kira Yukio Mukai Takeshi Noda Akitsugu Yamamoto Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae Biology Open Autophagy Unsaturated fatty acid OLE1 Fatty acid monodesaturation |
author_facet |
Yuta Ogasawara Shintaro Kira Yukio Mukai Takeshi Noda Akitsugu Yamamoto |
author_sort |
Yuta Ogasawara |
title |
Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae |
title_short |
Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae |
title_full |
Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae |
title_fullStr |
Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae |
title_full_unstemmed |
Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae |
title_sort |
ole1, fatty acid desaturase, is required for atg9 delivery and isolation membrane expansion during autophagy in saccharomyces cerevisiae |
publisher |
The Company of Biologists |
series |
Biology Open |
issn |
2046-6390 |
publishDate |
2017-01-01 |
description |
Macroautophagy, a major degradation pathway of cytoplasmic components, is carried out through formation of a double-membrane structure, the autophagosome. Although the involvement of specific lipid species in the formation process remains largely obscure, we recently showed that mono-unsaturated fatty acids (MUFA) generated by stearoyl-CoA desaturase 1 (SCD1) are required for autophagosome formation in mammalian cells. To obtain further insight into the role of MUFA in autophagy, in this study we analyzed the autophagic phenotypes of the yeast mutant of OLE1, an orthologue of SCD1. Δole1 cells were defective in nitrogen starvation-induced autophagy, and the Cvt pathway, when oleic acid was not supplied. Defects in elongation of the isolation membrane led to a defect in autophagosome formation. In the absence of Ole1, the transmembrane protein Atg9 was not able to reach the pre-autophagosomal structure (PAS), the site of autophagosome formation. Thus, autophagosome formation requires Ole1 during the delivery of Atg9 to the PAS/autophagosome from its cellular reservoir. |
topic |
Autophagy Unsaturated fatty acid OLE1 Fatty acid monodesaturation |
url |
http://bio.biologists.org/content/6/1/35 |
work_keys_str_mv |
AT yutaogasawara ole1fattyaciddesaturaseisrequiredforatg9deliveryandisolationmembraneexpansionduringautophagyinsaccharomycescerevisiae AT shintarokira ole1fattyaciddesaturaseisrequiredforatg9deliveryandisolationmembraneexpansionduringautophagyinsaccharomycescerevisiae AT yukiomukai ole1fattyaciddesaturaseisrequiredforatg9deliveryandisolationmembraneexpansionduringautophagyinsaccharomycescerevisiae AT takeshinoda ole1fattyaciddesaturaseisrequiredforatg9deliveryandisolationmembraneexpansionduringautophagyinsaccharomycescerevisiae AT akitsuguyamamoto ole1fattyaciddesaturaseisrequiredforatg9deliveryandisolationmembraneexpansionduringautophagyinsaccharomycescerevisiae |
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