Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae

Macroautophagy, a major degradation pathway of cytoplasmic components, is carried out through formation of a double-membrane structure, the autophagosome. Although the involvement of specific lipid species in the formation process remains largely obscure, we recently showed that mono-unsaturated fat...

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Main Authors: Yuta Ogasawara, Shintaro Kira, Yukio Mukai, Takeshi Noda, Akitsugu Yamamoto
Format: Article
Language:English
Published: The Company of Biologists 2017-01-01
Series:Biology Open
Subjects:
Online Access:http://bio.biologists.org/content/6/1/35
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spelling doaj-243e5b1e1fa243c78fcb64146d89df902021-06-02T19:22:37ZengThe Company of BiologistsBiology Open2046-63902017-01-0161354010.1242/bio.022053022053Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiaeYuta Ogasawara0Shintaro Kira1Yukio Mukai2Takeshi Noda3Akitsugu Yamamoto4 Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, Shiga 526-0829, Japan Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, Shiga 526-0829, Japan Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan Nagahama Institute of Bio-Science and Technology, 1266 Tamura, Nagahama, Shiga 526-0829, Japan Macroautophagy, a major degradation pathway of cytoplasmic components, is carried out through formation of a double-membrane structure, the autophagosome. Although the involvement of specific lipid species in the formation process remains largely obscure, we recently showed that mono-unsaturated fatty acids (MUFA) generated by stearoyl-CoA desaturase 1 (SCD1) are required for autophagosome formation in mammalian cells. To obtain further insight into the role of MUFA in autophagy, in this study we analyzed the autophagic phenotypes of the yeast mutant of OLE1, an orthologue of SCD1. Δole1 cells were defective in nitrogen starvation-induced autophagy, and the Cvt pathway, when oleic acid was not supplied. Defects in elongation of the isolation membrane led to a defect in autophagosome formation. In the absence of Ole1, the transmembrane protein Atg9 was not able to reach the pre-autophagosomal structure (PAS), the site of autophagosome formation. Thus, autophagosome formation requires Ole1 during the delivery of Atg9 to the PAS/autophagosome from its cellular reservoir.http://bio.biologists.org/content/6/1/35AutophagyUnsaturated fatty acidOLE1Fatty acid monodesaturation
collection DOAJ
language English
format Article
sources DOAJ
author Yuta Ogasawara
Shintaro Kira
Yukio Mukai
Takeshi Noda
Akitsugu Yamamoto
spellingShingle Yuta Ogasawara
Shintaro Kira
Yukio Mukai
Takeshi Noda
Akitsugu Yamamoto
Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae
Biology Open
Autophagy
Unsaturated fatty acid
OLE1
Fatty acid monodesaturation
author_facet Yuta Ogasawara
Shintaro Kira
Yukio Mukai
Takeshi Noda
Akitsugu Yamamoto
author_sort Yuta Ogasawara
title Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae
title_short Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae
title_full Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae
title_fullStr Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae
title_full_unstemmed Ole1, fatty acid desaturase, is required for Atg9 delivery and isolation membrane expansion during autophagy in Saccharomyces cerevisiae
title_sort ole1, fatty acid desaturase, is required for atg9 delivery and isolation membrane expansion during autophagy in saccharomyces cerevisiae
publisher The Company of Biologists
series Biology Open
issn 2046-6390
publishDate 2017-01-01
description Macroautophagy, a major degradation pathway of cytoplasmic components, is carried out through formation of a double-membrane structure, the autophagosome. Although the involvement of specific lipid species in the formation process remains largely obscure, we recently showed that mono-unsaturated fatty acids (MUFA) generated by stearoyl-CoA desaturase 1 (SCD1) are required for autophagosome formation in mammalian cells. To obtain further insight into the role of MUFA in autophagy, in this study we analyzed the autophagic phenotypes of the yeast mutant of OLE1, an orthologue of SCD1. Δole1 cells were defective in nitrogen starvation-induced autophagy, and the Cvt pathway, when oleic acid was not supplied. Defects in elongation of the isolation membrane led to a defect in autophagosome formation. In the absence of Ole1, the transmembrane protein Atg9 was not able to reach the pre-autophagosomal structure (PAS), the site of autophagosome formation. Thus, autophagosome formation requires Ole1 during the delivery of Atg9 to the PAS/autophagosome from its cellular reservoir.
topic Autophagy
Unsaturated fatty acid
OLE1
Fatty acid monodesaturation
url http://bio.biologists.org/content/6/1/35
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