Optimization of a DNA Nicking Assay to Evaluate Oenocarpus bataua and Camellia sinensis Antioxidant Capacity

Optimization of a DNA Nicking Assay to Evaluate Oenocarpus bataua and Camellia sinensis Antioxidant Capacity

This study was aimed at assessing the DNA damage protective activity of different types of extracts (aqueous, methanolic and acetonic) using an in vitro DNA nicking assay. Several parameters were optimized using the pUC18 plasmid, especially FeSO4, EDTA, solvent concentrations and incubation time. S...

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Bibliographic Details
Main Authors: Louis-Jérôme Leba, Christel Brunschwig, Mona Saout, Karine Martial, Emmanuelle Vulcain, Didier Bereau, Jean-Charles Robinson
Format: Article
Language:English
Published: MDPI AG 2014-10-01
Series:International Journal of Molecular Sciences
Subjects:
pUC18
antioxidant
prooxidant
DNA nicking assay
Fenton
Amazonian palm
Oenocarpus bataua
Camellia sinensis
Online Access:http://www.mdpi.com/1422-0067/15/10/18023

Internet

http://www.mdpi.com/1422-0067/15/10/18023

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