Pengaruh Media Tumbuh terhadap Kadar Protein Saccharomyces cerevisiae dalam Pembuatan Protein Sel Tunggal

• Main Authors: RATNA SETYANINGSIH, ERNA PURWITASARI, ARTINI PANGASTUTI
• Format: Article
• Language: English
• Published: MBI & UNS Solo 2004-11-01
• Series: Bioteknologi
• Subjects: The aim of this research was to examine the influence of difference growth media; i. e. tofu liquid waste; tofu solid waste; and coconut water in various composition and Yeast Extract Peptone Dextrose (YEPD); to protein contents of Saccharomyces cerevisiae in Single Cell Protein (SCP) production. The framework of this research was that tofu liquid waste; and coconut water were containing a lot of carbons; nitrogens; minerals; and vitamin that could be used as growth medium of S. cerevisiae to produce SCP; which was commonly used. The medium from tofu liquid waste and the coconut water were made by ratio 2:1; 1:1; 1:2 and added with tofu solid waste 1.5 g and 2.5 g. Then; the measurement of pH medium; the amount of cell; cell dried weight; and the protein content in S. cerevisiae was done. The measurement of protein content was done by Lowry method. The result of the research showed that growth media influenced protein content of S. cerevisiae. Protein content of S. cerevisiae cultured in tofu liquid waste- coconut water was lower then in YEPD medium. The protein content of S. cerevisiae cultured in tofu liquid waste and coconut water ratio 1:2; added with 2.5 g tofu solid waste was higher then in other medium composition
• Online Access: http://biosains.mipa.uns.ac.id/C/C0102/C010202.pdf

The aim of this research was to examine the influence of difference growth media, i. e. tofu liquid waste, tofu solid waste, and coconut water in various composition and Yeast Extract Peptone Dextrose (YEPD), to protein contents of Saccharomyces cerevisiae in Single Cell Protein (SCP) production. The framework of this research was that tofu liquid waste, tofu solid waste, and coconut water were containing a lot of carbons, nitrogens, minerals, and vitamin that could be used as growth medium of S. cerevisiae to produce SCP, which was commonly used. The medium from tofu liquid waste and the coconut water were made by ratio 2:1, 1:1, 1:2 and added with tofu solid waste 1.5 g and 2.5 g. Then, the measurement of pH medium, the amount of cell, cell dried weight, and the protein content in S. cerevisiae was done. The measurement of protein content was done by Lowry method. The result of the research showed that growth media influenced protein content of S. cerevisiae. Protein content of S. cerevisiae cultured in tofu liquid waste- coconut water was lower then in YEPD medium. The protein content of S. cerevisiae cultured in tofu liquid waste and coconut water ratio 1:2, added with 2.5 g tofu solid waste was higher then in other medium composition.