Rosemary extract reverses oxidative stress through activation of Nrf2 signaling pathway in hamsters fed on high fat diet and HepG2 cells

Rosemary extract reverses oxidative stress through activation of Nrf2 signaling pathway in hamsters fed on high fat diet and HepG2 cells

Rosemary extract (RE), a useful component isolated from Rosmarinus officinalis L., was recently reported to protect cells against the harmful effects of ROS. However, the underlying mechanisms of this action remained unknown. We investigated the defensive effect of RE on the oxidative stress injury...

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Main Authors: Huali Wang, Jing Cheng, Shengnan Yang, Steve W. Cui, Mingchun Wang, Wang Hao
Format: Article
Language:English
Published: Elsevier 2020-11-01
Series:Journal of Functional Foods
Subjects:
Rosemary extract
Oxidative stress
High fat diet
Oleic acid
NF-E2-related factor 2
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464620303601
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spelling doaj-23dc11a20a4d45c98cfbb8cb72292bbb2021-04-30T07:19:32ZengElsevierJournal of Functional Foods1756-46462020-11-0174104136Rosemary extract reverses oxidative stress through activation of Nrf2 signaling pathway in hamsters fed on high fat diet and HepG2 cellsHuali Wang0Jing Cheng1Shengnan Yang2Steve W. Cui3Mingchun Wang4Wang Hao5China National Center for Food Safety Risk Assessment, Beijing 100022, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, ChinaGuelph Research and Development Centre, Agriculture and Agri-Food Canada, 93 Stone Road W., Guelph N1G 5C9, CanadaCollege of Tea and Food Science and Technology, Anhui agricultural university, Anhui 230036, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), Tianjin 300457, China; Corresponding author.Rosemary extract (RE), a useful component isolated from Rosmarinus officinalis L., was recently reported to protect cells against the harmful effects of ROS. However, the underlying mechanisms of this action remained unknown. We investigated the defensive effect of RE on the oxidative stress injury resulted from high fat diet in hamsters and oleic acid (OA) in HepG2 cells respectively, and its underlying mechanisms. Treatment with RE alleviated liver steatosis and reduced plasma and liver content of TG and TC, as well as plasma content of AST and ALT. RE apparently increased the activity of SOD, GSH-Px, CAT and decreased MDA content. RE notably increased CuZn-SOD, Mn-SOD, CAT, GSH-Px, GCLC, GCLM and Nrf2 mRNA and protein expression level. Nrf2 shRNA knockdown in HepG2 cells increased OA-induced ROS generation, and effectively altered OA-induced cytotoxicity, suggesting that Nrf2 is a crucial mediator against high-fat induced oxidative damage.http://www.sciencedirect.com/science/article/pii/S1756464620303601Rosemary extractOxidative stressHigh fat dietOleic acidNF-E2-related factor 2
collection DOAJ
language English
format Article
sources DOAJ
author Huali Wang
Jing Cheng
Shengnan Yang
Steve W. Cui
Mingchun Wang
Wang Hao
spellingShingle Huali Wang
Jing Cheng
Shengnan Yang
Steve W. Cui
Mingchun Wang
Wang Hao
Rosemary extract reverses oxidative stress through activation of Nrf2 signaling pathway in hamsters fed on high fat diet and HepG2 cells
Journal of Functional Foods
Rosemary extract
Oxidative stress
High fat diet
Oleic acid
NF-E2-related factor 2
author_facet Huali Wang
Jing Cheng
Shengnan Yang
Steve W. Cui
Mingchun Wang
Wang Hao
author_sort Huali Wang
title Rosemary extract reverses oxidative stress through activation of Nrf2 signaling pathway in hamsters fed on high fat diet and HepG2 cells
title_short Rosemary extract reverses oxidative stress through activation of Nrf2 signaling pathway in hamsters fed on high fat diet and HepG2 cells
title_full Rosemary extract reverses oxidative stress through activation of Nrf2 signaling pathway in hamsters fed on high fat diet and HepG2 cells
title_fullStr Rosemary extract reverses oxidative stress through activation of Nrf2 signaling pathway in hamsters fed on high fat diet and HepG2 cells
title_full_unstemmed Rosemary extract reverses oxidative stress through activation of Nrf2 signaling pathway in hamsters fed on high fat diet and HepG2 cells
title_sort rosemary extract reverses oxidative stress through activation of nrf2 signaling pathway in hamsters fed on high fat diet and hepg2 cells
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2020-11-01
description Rosemary extract (RE), a useful component isolated from Rosmarinus officinalis L., was recently reported to protect cells against the harmful effects of ROS. However, the underlying mechanisms of this action remained unknown. We investigated the defensive effect of RE on the oxidative stress injury resulted from high fat diet in hamsters and oleic acid (OA) in HepG2 cells respectively, and its underlying mechanisms. Treatment with RE alleviated liver steatosis and reduced plasma and liver content of TG and TC, as well as plasma content of AST and ALT. RE apparently increased the activity of SOD, GSH-Px, CAT and decreased MDA content. RE notably increased CuZn-SOD, Mn-SOD, CAT, GSH-Px, GCLC, GCLM and Nrf2 mRNA and protein expression level. Nrf2 shRNA knockdown in HepG2 cells increased OA-induced ROS generation, and effectively altered OA-induced cytotoxicity, suggesting that Nrf2 is a crucial mediator against high-fat induced oxidative damage.
topic Rosemary extract
Oxidative stress
High fat diet
Oleic acid
NF-E2-related factor 2
url http://www.sciencedirect.com/science/article/pii/S1756464620303601
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