Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies

New analytics of post-transcriptional RNA modifications have paved the way for a tremendous upswing of the biological and biomedical research in this field. This especially applies to methods that included RNA-Seq techniques, and which typically result in what is termed global scale modification map...

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Main Authors: Yuri Motorin, Mark Helm
Format: Article
Language:English
Published: MDPI AG 2019-01-01
Series:Genes
Subjects:
Online Access:http://www.mdpi.com/2073-4425/10/1/35
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spelling doaj-23ccd6c066c0476e87b1c1da304e19ee2020-11-24T23:02:02ZengMDPI AGGenes2073-44252019-01-011013510.3390/genes10010035genes10010035Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging TechnologiesYuri Motorin0Mark Helm1Laboratoire IMoPA, UMR7365 National Centre for Scientific Research (CNRS)-Lorraine University, Biopôle, 9 Avenue de la Forêt de Haye, 54505 Vandoeuvre-les-Nancy, FranceInstitute of Pharmacy and Biochemistry, Johannes Gutenberg-University Mainz, Staudingerweg 5, 55128 Mainz, GermanyNew analytics of post-transcriptional RNA modifications have paved the way for a tremendous upswing of the biological and biomedical research in this field. This especially applies to methods that included RNA-Seq techniques, and which typically result in what is termed global scale modification mapping. In this process, positions inside a cell`s transcriptome are receiving a status of potential modification sites (so called modification calling), typically based on a score of some kind that issues from the particular method applied. The resulting data are thought to represent information that goes beyond what is contained in typical transcriptome data, and hence the field has taken to use the term “epitranscriptome”. Due to the high rate of newly published mapping techniques, a significant number of chemically distinct RNA modifications have become amenable to mapping, albeit with variegated accuracy and precision, depending on the nature of the technique. This review gives a brief overview of known techniques, and how they were applied to modification calling.http://www.mdpi.com/2073-4425/10/1/35RNA modificationepitranscriptomedeep sequencingNext Generation SequencingRNA-Seqchemical treatmentengineered Reverse Transcriptase enzymes
collection DOAJ
language English
format Article
sources DOAJ
author Yuri Motorin
Mark Helm
spellingShingle Yuri Motorin
Mark Helm
Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies
Genes
RNA modification
epitranscriptome
deep sequencing
Next Generation Sequencing
RNA-Seq
chemical treatment
engineered Reverse Transcriptase enzymes
author_facet Yuri Motorin
Mark Helm
author_sort Yuri Motorin
title Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies
title_short Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies
title_full Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies
title_fullStr Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies
title_full_unstemmed Methods for RNA Modification Mapping Using Deep Sequencing: Established and New Emerging Technologies
title_sort methods for rna modification mapping using deep sequencing: established and new emerging technologies
publisher MDPI AG
series Genes
issn 2073-4425
publishDate 2019-01-01
description New analytics of post-transcriptional RNA modifications have paved the way for a tremendous upswing of the biological and biomedical research in this field. This especially applies to methods that included RNA-Seq techniques, and which typically result in what is termed global scale modification mapping. In this process, positions inside a cell`s transcriptome are receiving a status of potential modification sites (so called modification calling), typically based on a score of some kind that issues from the particular method applied. The resulting data are thought to represent information that goes beyond what is contained in typical transcriptome data, and hence the field has taken to use the term “epitranscriptome”. Due to the high rate of newly published mapping techniques, a significant number of chemically distinct RNA modifications have become amenable to mapping, albeit with variegated accuracy and precision, depending on the nature of the technique. This review gives a brief overview of known techniques, and how they were applied to modification calling.
topic RNA modification
epitranscriptome
deep sequencing
Next Generation Sequencing
RNA-Seq
chemical treatment
engineered Reverse Transcriptase enzymes
url http://www.mdpi.com/2073-4425/10/1/35
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