Testing of newly developed glycophospholipid antigen for the detection of P. falciparum malaria by laser light immunoassay in endemic and non-endemic areas

• Main Author: A. Roy, S. Biswas, M.M. Mya, R.K. Saxena & K.B. Roy
• Format: Article
• Language: English
• Published: Wolters Kluwer Medknow Publications 2003-09-01
• Series: Journal of Vector Borne Diseases
• Subjects: Laser light immunoassay; microscopic examination; per cent positivity; Pf malaria
• Online Access: http://www.mrcindia.org/journal/issues/403105.pdf

A glycophospholipid (GPL) antigen isolated from Plasmodium falciparum culture supernatant hasbeen tested for its antigenicity. Detection of malaria positive known blood samples and unknown fieldsamples from endemic and non-endemic areas were compared. In this study laser light scattering immunoassay(LIA) was used for the detection of P. falciparum malaria. Test results of control (malaria negativesamples from Surat) were compared with known positive samples and unknown malaria positivefield samples. A positive correlation has been observed (97%) in falciparum positive samples from laboratoryand unknown samples from endemic area (Haldwani) by LIA method using GPL antigen. Fromthe results of the study it was found that GPL antigen has a better antigenic property and can detectalmost all the cases of Pf malaria by LIA method.