CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK

CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK

We studied the viability and dynamic of cell distribution during long-term cultivation of broblasts 3T3 in spider silk spidroin 1-based scaffold. Laser scanning confocal microscopy is shown to have advantages for visualization of cells situated on the external and internal surfaces of scaffold. Fibr...

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Main Authors: O. L. Pustovalova, I. I. Agapov, M. M. Moisenovich, P. S. Eremin, A. A. Kazulina, A. Y. Arhipova, A. A. Ramonova, V. G. Bogush, V. I. Sevastianov, M. P. Kirpichnikov
Format: Article
Language:Russian
Published: Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov 2009-05-01
Series:Vestnik Transplantologii i Iskusstvennyh Organov
Subjects:
tissue engineering
recombinant spider silk
Online Access:https://journal.transpl.ru/vtio/article/view/233
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spelling doaj-23b471ea0aa94b3f905c7a9fcd8fecb62021-07-29T09:08:27ZrusFederal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov Vestnik Transplantologii i Iskusstvennyh Organov1995-11912009-05-01112545910.15825/1995-1191-2009-2-54-59176CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILKO. L. Pustovalova0I. I. Agapov1M. M. Moisenovich2P. S. Eremin3A. A. Kazulina4A. Y. Arhipova5A. A. Ramonova6V. G. Bogush7V. I. Sevastianov8M. P. Kirpichnikov9Moscow State University, biological facultyAcademician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, MoscowMoscow State University, biological facultyMoscow State University, biological facultyMoscow State University, biological facultyMoscow State University, biological facultyMoscow State University, biological facultyState Scientific Center «GNIIgenetika»Academician V.I. Shumakov Federal Research Center of Transplantology and Artificial Organs, MoscowMoscow State University, biological facultyWe studied the viability and dynamic of cell distribution during long-term cultivation of broblasts 3T3 in spider silk spidroin 1-based scaffold. Laser scanning confocal microscopy is shown to have advantages for visualization of cells situated on the external and internal surfaces of scaffold. Fibroblasts maintain high proliferative ability and viability during long term cultivation. Spidroin 1-based scaffold are the perspective materials for bioengineering.https://journal.transpl.ru/vtio/article/view/233tissue engineeringrecombinant spider silk
collection DOAJ
language Russian
format Article
sources DOAJ
author O. L. Pustovalova
I. I. Agapov
M. M. Moisenovich
P. S. Eremin
A. A. Kazulina
A. Y. Arhipova
A. A. Ramonova
V. G. Bogush
V. I. Sevastianov
M. P. Kirpichnikov
spellingShingle O. L. Pustovalova
I. I. Agapov
M. M. Moisenovich
P. S. Eremin
A. A. Kazulina
A. Y. Arhipova
A. A. Ramonova
V. G. Bogush
V. I. Sevastianov
M. P. Kirpichnikov
CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
Vestnik Transplantologii i Iskusstvennyh Organov
tissue engineering
recombinant spider silk
author_facet O. L. Pustovalova
I. I. Agapov
M. M. Moisenovich
P. S. Eremin
A. A. Kazulina
A. Y. Arhipova
A. A. Ramonova
V. G. Bogush
V. I. Sevastianov
M. P. Kirpichnikov
author_sort O. L. Pustovalova
title CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_short CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_full CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_fullStr CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_full_unstemmed CONFOCAL MICROSCOPY STUDY OF BIOLOGICAL PECULIARITIES OF SCAFFOLD MADE FROM RECOMBINANT SPIDER SILK
title_sort confocal microscopy study of biological peculiarities of scaffold made from recombinant spider silk
publisher Federal Research Center of Transplantology and Artificial Organs named after V.I.Shumakov
series Vestnik Transplantologii i Iskusstvennyh Organov
issn 1995-1191
publishDate 2009-05-01
description We studied the viability and dynamic of cell distribution during long-term cultivation of broblasts 3T3 in spider silk spidroin 1-based scaffold. Laser scanning confocal microscopy is shown to have advantages for visualization of cells situated on the external and internal surfaces of scaffold. Fibroblasts maintain high proliferative ability and viability during long term cultivation. Spidroin 1-based scaffold are the perspective materials for bioengineering.
topic tissue engineering
recombinant spider silk
url https://journal.transpl.ru/vtio/article/view/233
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