Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression system

Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression system

Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) are cytokines widely used in ex vivo monocyte differentiation experiments, vaccine formulations and disease treatment. The aim of this study was to produce recombinant bovine GM-CSF and IL-4 in an episomal expression...

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Bibliographic Details
Main Authors: Blanco Federico Carlos, Vazquez Cristina Lourdes, García Julia Sabio y, Rocha Rosana Valeria, Gravisaco María José, Forrellad Marina Andrea, Magistrelli Giovanni, Bigi Fabiana
Format: Article
Language:English
Published: Sciendo 2021-08-01
Series:Journal of Veterinary Research
Subjects:
bovine gm-csf
bovine il-4
dendritic cells
macrophages
mammalian episomal expression
Online Access:https://doi.org/10.2478/jvetres-2021-0043
Description
Summary:Granulocyte-macrophage colony stimulating factor (GM-CSF) and interleukin-4 (IL-4) are cytokines widely used in ex vivo monocyte differentiation experiments, vaccine formulations and disease treatment. The aim of this study was to produce recombinant bovine GM-CSF and IL-4 in an episomal expression system that conserves the postransductional modification of the native proteins and to use the products to differentiate bovine monocytes into dendritic cells.
ISSN:2450-8608

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