Analysis of Expressed Sequence Tags from Chinese Bayberry Fruit (Myrica rubra Sieb. and Zucc.) at Different Ripening Stages and Their Association with Fruit Quality Development

• Main Authors: Kunsong Chen, Chongde Sun, Changjie Xu, Xian Li, Chao Feng, Changqing Zhu
• Format: Article
• Language: English
• Published: MDPI AG 2013-02-01
• Series: International Journal of Molecular Sciences
• Subjects: Chinese bayberry (Myrica rubra Sieb and Zucc.); fruit ripening; expressed sequence tags (EST); fruit quality; development; senescence
• Online Access: http://www.mdpi.com/1422-0067/14/2/3110
• ISSN: 1422-0067

A total of 2000 EST sequences were produced from cDNA libraries generated from Chinese bayberry fruit (Myrica rubra Sieb. and Zucc. cv. “Biqi”) at four different ripening stages. After cluster and assembly analysis of the datasets by UniProt, 395 unigenes were identified, and their presumed functions were assigned to 14 putative cellular roles. Furthermore, a sequence BLAST was done for the top ten highly expressed genes in the ESTs, and genes associated with disease/defense and anthocyanin accumulation were analyzed. Gene-encoding elements associated with ethylene biosynthesis and signal transductions, in addition to other senescence-regulating proteins, as well as those associated with quality formation during fruit ripening, were also identified. Their possible roles were subsequently discussed.