A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology

A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology

Tools that allow for rapid, accurate and inexpensive assembly of multi-component combinatorial libraries of DNA for transformation into plants will accelerate the progress of synthetic biology research. Recent progress in molecular cloning methods has vastly expanded the repertoire with which plant...

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Bibliographic Details
Main Authors: Shahram eEmami, Muh-ching eYee, Jose R. Dinneny
Format: Article
Language:English
Published: Frontiers Media S.A. 2013-09-01
Series:Frontiers in Plant Science
Subjects:
Synthetic Biology
Molecular cloning
plant transformation
Agrobacterium binary vectors
combinatorial libraries
Golden Gate Cloning
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00339/full
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spelling doaj-236fd203893b4f7ca1c0b721f019d01b2020-11-24T22:48:13ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2013-09-01410.3389/fpls.2013.0033961735A robust family of Golden Gate Agrobacterium vectors for plant synthetic biologyShahram eEmami0Muh-ching eYee1Jose R. Dinneny2Carnegie Institution for ScienceCarnegie Institution for ScienceCarnegie Institution for ScienceTools that allow for rapid, accurate and inexpensive assembly of multi-component combinatorial libraries of DNA for transformation into plants will accelerate the progress of synthetic biology research. Recent progress in molecular cloning methods has vastly expanded the repertoire with which plant biologists can engineer a transgene. Here we describe a new set of binary vectors for use in Agrobacterium-mediated plant transformation that utilizes the Golden-Gate cloning approach. Our optimized protocol facilitates the rapid and inexpensive generation of multi-component transgenes for later introduction into plants.http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00339/fullSynthetic BiologyMolecular cloningplant transformationAgrobacterium binary vectorscombinatorial librariesGolden Gate Cloning
collection DOAJ
language English
format Article
sources DOAJ
author Shahram eEmami
Muh-ching eYee
Jose R. Dinneny
spellingShingle Shahram eEmami
Muh-ching eYee
Jose R. Dinneny
A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
Frontiers in Plant Science
Synthetic Biology
Molecular cloning
plant transformation
Agrobacterium binary vectors
combinatorial libraries
Golden Gate Cloning
author_facet Shahram eEmami
Muh-ching eYee
Jose R. Dinneny
author_sort Shahram eEmami
title A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_short A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_full A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_fullStr A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_full_unstemmed A robust family of Golden Gate Agrobacterium vectors for plant synthetic biology
title_sort robust family of golden gate agrobacterium vectors for plant synthetic biology
publisher Frontiers Media S.A.
series Frontiers in Plant Science
issn 1664-462X
publishDate 2013-09-01
description Tools that allow for rapid, accurate and inexpensive assembly of multi-component combinatorial libraries of DNA for transformation into plants will accelerate the progress of synthetic biology research. Recent progress in molecular cloning methods has vastly expanded the repertoire with which plant biologists can engineer a transgene. Here we describe a new set of binary vectors for use in Agrobacterium-mediated plant transformation that utilizes the Golden-Gate cloning approach. Our optimized protocol facilitates the rapid and inexpensive generation of multi-component transgenes for later introduction into plants.
topic Synthetic Biology
Molecular cloning
plant transformation
Agrobacterium binary vectors
combinatorial libraries
Golden Gate Cloning
url http://journal.frontiersin.org/Journal/10.3389/fpls.2013.00339/full
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