Whole genome sequence analysis of multi drug resistant community associated methicillin resistant Staphylococcus aureus from food fish: detection of clonal lineage ST 28 and its antimicrobial resistance and virulence genes

• Main Authors: Gopalan Krishnan Sivaraman, Visnuvinayagam Sivam, Balasubramanian Ganesh, Ravikrishnan Elangovan, Ardhra Vijayan, Mukteswar Prasad Mothadaka
• Format: Article
• Language: English
• Published: PeerJ Inc. 2021-05-01
• Series: PeerJ
• Subjects: Whole genome sequence; Virulence genes; Methicillin resistant Staphylococcus aureus; Antimicrobial resistance genes; Fish
• Online Access: https://peerj.com/articles/11224.pdf

Methicillin-resistant staphylococcus aureus (MRSA) sequence type 28 (ST 28) and spa type t021 is a CC30, prototype of ST-30, Community Associated-MRSA (CA-MRSA) (lukS-lukF +). It is a multi-drug resistant strain harbouring staphylococcal endotoxins, haemolysins, ureolysin, serine protease, and antimicrobial resistance genes. In this study, we report the draft genome sequence of this MRSA isolated from the most commonly used food fish, ribbon fish (Trichiurus lepturus). The total number of assembled paired-end high-quality reads was 7,731,542 with a total length of  2.8Mb of 2797 predicted genes. The unique ST28/ t021 CA- MRSA in fish is the first report from India, and in addition to antibiotic resistance is known to co-harbour virulence genes, haemolysins, aureolysins and endotoxins. Comprehensive comparative genomic analysis of CA-MRSA strain7 can help further understand their diversity, genetic structure, diversity and a high degree of virulence to aid in fisheries management.