Influence of breed on the clinical and hemato-biochemical parameters in sheep experimentally infected with Leptospira sp.
Early diagnosis of leptospirosis may aid in a favorable prognosis in infected animals, but there are few reports of clinical and hematochemical changes in the ovine species, nor whether the breed exerts any influence on the response to infection. Thus, the objective of the present study was to evalu...
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Format: | Article |
Language: | English |
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Elsevier
2019-10-01
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Series: | Heliyon |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844019363807 |
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doaj-233b27db4262482aaf2f76179fff3212 |
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record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Diego Figueiredo da Costa Pedro Jorge Àlvares de Faria Denise Batista Nogueira Laura Honório de Oliveira Tolentino Maira Pôrto Viana José Devedê da Silva Antônio Fernando de Melo Vaz Severino Silvano dos Santos Higino Sergio Santos de Azevedo Clebert José Alves |
spellingShingle |
Diego Figueiredo da Costa Pedro Jorge Àlvares de Faria Denise Batista Nogueira Laura Honório de Oliveira Tolentino Maira Pôrto Viana José Devedê da Silva Antônio Fernando de Melo Vaz Severino Silvano dos Santos Higino Sergio Santos de Azevedo Clebert José Alves Influence of breed on the clinical and hemato-biochemical parameters in sheep experimentally infected with Leptospira sp. Heliyon Agriculture Environmental science Microbiology Leptospirosis Intraperitoneal route Conjunctival route |
author_facet |
Diego Figueiredo da Costa Pedro Jorge Àlvares de Faria Denise Batista Nogueira Laura Honório de Oliveira Tolentino Maira Pôrto Viana José Devedê da Silva Antônio Fernando de Melo Vaz Severino Silvano dos Santos Higino Sergio Santos de Azevedo Clebert José Alves |
author_sort |
Diego Figueiredo da Costa |
title |
Influence of breed on the clinical and hemato-biochemical parameters in sheep experimentally infected with Leptospira sp. |
title_short |
Influence of breed on the clinical and hemato-biochemical parameters in sheep experimentally infected with Leptospira sp. |
title_full |
Influence of breed on the clinical and hemato-biochemical parameters in sheep experimentally infected with Leptospira sp. |
title_fullStr |
Influence of breed on the clinical and hemato-biochemical parameters in sheep experimentally infected with Leptospira sp. |
title_full_unstemmed |
Influence of breed on the clinical and hemato-biochemical parameters in sheep experimentally infected with Leptospira sp. |
title_sort |
influence of breed on the clinical and hemato-biochemical parameters in sheep experimentally infected with leptospira sp. |
publisher |
Elsevier |
series |
Heliyon |
issn |
2405-8440 |
publishDate |
2019-10-01 |
description |
Early diagnosis of leptospirosis may aid in a favorable prognosis in infected animals, but there are few reports of clinical and hematochemical changes in the ovine species, nor whether the breed exerts any influence on the response to infection. Thus, the objective of the present study was to evaluate the clinical, biochemical and hematological alterations in Santa Inês and crossbred ewes challenged with Leptospira interrogans serogroup Pomona serovar Kennewicki. Twenty-four sheep were used in this experiment, 12 crossbred (group A) and 12 Santa Inês (group B). In each group, sheep were conjunctivally and intraperitoneally challenged. During 60 days post-infection the ewes were evaluated for the presentation of clinical signs and the blood was collected for hemogram and serum biochemistry. Concentration of urea and creatinine; serum aspartate aminotransferase activity (AST); gamma-glutamyltransferase (GGT); total protein and albumin; total bilirubin, direct and indirect were analyzed. The urine of these sheep was collected for urinalysis. Only two Santa Inês sheep showed blood in the urine. Clinical signs implicated in Leptospira sp. infection were not identified. Some sheep had anemia, especially crossbred. However, anemia may be attributed to the more effective cellular response that has been identified in crossbred animals. Only one animal presented leukocytosis with neutrophilia, while 11 presented atypical leukopenia, especially those of the Santa Inês breed. There was a decrease in total protein and albumin, as well as the increase in gammaglutamyltranspeptidase (GGT), especially in Santa Inês sheep. The level of aspartate aminotransferase (AST) remained within the normal range for the species. A significant increase (p < 0.05) in conjugated bilirubin levels in challenged animals was detected. Only one sheep intraperitoneally challenged presented a high level of urea in the blood, but the creatinine level remained within the normal range. The intraperitoneal route was responsible for more significant changes (p < 0.05) in the hemogram and biochemistry when compared to the conjunctival route. The results indicate that crossbred sheep have a more efficient cellular response than Santa Inês sheep, which may confer a greater resistance to infection. Clinical signs are not good parameters to follow the development of leptospirosis in crossbred and Santa Inês breed. Hematological and biochemical analyzes were useful in the detection of anemia and possible liver changes caused by leptospirosis. The intraperitoneal route was able to cause more conclusive alterations of the analyzed parameters, however, it is possible that the alterations caused by the conjunctival route reproduce in a more faithful way what happens in a natural situation of infection. |
topic |
Agriculture Environmental science Microbiology Leptospirosis Intraperitoneal route Conjunctival route |
url |
http://www.sciencedirect.com/science/article/pii/S2405844019363807 |
work_keys_str_mv |
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spelling |
doaj-233b27db4262482aaf2f76179fff32122020-11-25T02:00:29ZengElsevierHeliyon2405-84402019-10-01510e02720Influence of breed on the clinical and hemato-biochemical parameters in sheep experimentally infected with Leptospira sp.Diego Figueiredo da Costa0Pedro Jorge Àlvares de Faria1Denise Batista Nogueira2Laura Honório de Oliveira Tolentino3Maira Pôrto Viana4José Devedê da Silva5Antônio Fernando de Melo Vaz6Severino Silvano dos Santos Higino7Sergio Santos de Azevedo8Clebert José Alves9Transmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilTransmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilTransmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilTransmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilTransmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilTransmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilTransmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilTransmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilTransmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilCorresponding author.; Transmissible Diseases Laboratory, Universidade Federal de Campina Grande (UFCG), Av. Universitária, s/n, Santa Cecília, 58700-970, Patos, PB, BrazilEarly diagnosis of leptospirosis may aid in a favorable prognosis in infected animals, but there are few reports of clinical and hematochemical changes in the ovine species, nor whether the breed exerts any influence on the response to infection. Thus, the objective of the present study was to evaluate the clinical, biochemical and hematological alterations in Santa Inês and crossbred ewes challenged with Leptospira interrogans serogroup Pomona serovar Kennewicki. Twenty-four sheep were used in this experiment, 12 crossbred (group A) and 12 Santa Inês (group B). In each group, sheep were conjunctivally and intraperitoneally challenged. During 60 days post-infection the ewes were evaluated for the presentation of clinical signs and the blood was collected for hemogram and serum biochemistry. Concentration of urea and creatinine; serum aspartate aminotransferase activity (AST); gamma-glutamyltransferase (GGT); total protein and albumin; total bilirubin, direct and indirect were analyzed. The urine of these sheep was collected for urinalysis. Only two Santa Inês sheep showed blood in the urine. Clinical signs implicated in Leptospira sp. infection were not identified. Some sheep had anemia, especially crossbred. However, anemia may be attributed to the more effective cellular response that has been identified in crossbred animals. Only one animal presented leukocytosis with neutrophilia, while 11 presented atypical leukopenia, especially those of the Santa Inês breed. There was a decrease in total protein and albumin, as well as the increase in gammaglutamyltranspeptidase (GGT), especially in Santa Inês sheep. The level of aspartate aminotransferase (AST) remained within the normal range for the species. A significant increase (p < 0.05) in conjugated bilirubin levels in challenged animals was detected. Only one sheep intraperitoneally challenged presented a high level of urea in the blood, but the creatinine level remained within the normal range. The intraperitoneal route was responsible for more significant changes (p < 0.05) in the hemogram and biochemistry when compared to the conjunctival route. The results indicate that crossbred sheep have a more efficient cellular response than Santa Inês sheep, which may confer a greater resistance to infection. Clinical signs are not good parameters to follow the development of leptospirosis in crossbred and Santa Inês breed. Hematological and biochemical analyzes were useful in the detection of anemia and possible liver changes caused by leptospirosis. The intraperitoneal route was able to cause more conclusive alterations of the analyzed parameters, however, it is possible that the alterations caused by the conjunctival route reproduce in a more faithful way what happens in a natural situation of infection.http://www.sciencedirect.com/science/article/pii/S2405844019363807AgricultureEnvironmental scienceMicrobiologyLeptospirosisIntraperitoneal routeConjunctival route |