Auto-FPFA: An Automated Microscope for Characterizing Genetically Encoded Biosensors

Abstract Genetically encoded biosensors function by linking structural change in a protein construct, typically tagged with one or more fluorescent proteins, to changes in a biological parameter of interest (such as calcium concentration, pH, phosphorylation-state, etc.). Typically, the structural c...

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Bibliographic Details
Main Authors: Tuan A. Nguyen, Henry L. Puhl, An K. Pham, Steven S. Vogel
Format: Article
Language:English
Published: Nature Publishing Group 2018-05-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-018-25689-x