Super-resolution fluorescence imaging and correlation spectroscopy: Principles and examples of application
Self-organization of cell-surface receptors in structurally distinct domains in the plasma membrane is of vital interest for correct cellular signaling. However, this dynamic process is difficult to study in cells with sufficiently high temporal and spatial resolution. We present here two...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Serbian Chemical Society
2013-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2013/0352-51391300102J.pdf |
| Summary: | Self-organization of cell-surface receptors in structurally distinct domains
in the plasma membrane is of vital interest for correct cellular signaling.
However, this dynamic process is difficult to study in cells with
sufficiently high temporal and spatial resolution. We present here two
quantitative high-resolution methods with single-molecule sensitivity,
Fluorescence Correlation Spectroscopy (FCS) and pair-correlation
Photoactivated Localization Microscopy (pcPALM), which enable nondestructive
study of receptor diffusion and lateral organization at the nanoscale level.
We introduce here the methods and review their application in studies of
lateral organization of G Protein-Coupled Receptors (GPCRs). Examples from
our own work on opioid receptor lateral organization are presented in order
to illustrate the most recent advances in the field. [Projekat Ministarstva
nauke Republike Srbije, br. 172015 i br. 45001] |
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| ISSN: | 0352-5139 1820-7421 |