Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region

Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region

In our previous study, we described the development of an internal transcribed spacer (ITS)1 sequencing method, and used this protocol in species-identification of isolated fungi collected from the manufacturing areas of a compounding company known to have caused the multistate fungal meningitis out...

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Bibliographic Details
Main Authors: Irshad M. Sulaiman, Emily Jacobs, Steven Simpson, Khalil Kerdahi
Format: Article
Language:English
Published: MDPI AG 2014-06-01
Series:Pathogens
Subjects:
nucleotide sequencing
rapid identification
foodborne disease
Online Access:http://www.mdpi.com/2076-0817/3/3/499
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spelling doaj-23029d3f0e5a484189f162409557d3b62020-11-25T01:28:24ZengMDPI AGPathogens2076-08172014-06-013349950910.3390/pathogens3030499pathogens3030499Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer RegionIrshad M. Sulaiman0Emily Jacobs1Steven Simpson2Khalil Kerdahi3Southeast Regional Laboratory, U.S. Food and Drug Administration, 60, Eighth Street NE, Atlanta, GA 30309, USASoutheast Regional Laboratory, U.S. Food and Drug Administration, 60, Eighth Street NE, Atlanta, GA 30309, USASoutheast Regional Laboratory, U.S. Food and Drug Administration, 60, Eighth Street NE, Atlanta, GA 30309, USASoutheast Regional Laboratory, U.S. Food and Drug Administration, 60, Eighth Street NE, Atlanta, GA 30309, USAIn our previous study, we described the development of an internal transcribed spacer (ITS)1 sequencing method, and used this protocol in species-identification of isolated fungi collected from the manufacturing areas of a compounding company known to have caused the multistate fungal meningitis outbreak in the United States. In this follow-up study, we have analyzed the unopened vials of Greek yogurt from the recalled batch to determine the possible cause of microbial contamination in the product. A total of 15 unopened vials of Greek yogurt belonging to the recalled batch were examined for the detection of fungi in these samples known to cause foodborne illness following conventional microbiological protocols. Fungi were isolated from all of the 15 Greek yogurt samples analyzed. The isolated fungi were genetically typed by DNA sequencing of PCR-amplified ITS1 region of rRNA gene. Analysis of data confirmed all of the isolated fungal isolates from the Greek yogurt to be Rhizomucor variabilis. The generated ITS1 sequences matched 100% with the published sequences available in GenBank. In addition, these yogurt samples were also tested for the presence of five types of bacteria (Salmonella, Listeria, Staphylococcus, Bacillus and Escherichia coli) causing foodborne disease in humans, and found negative for all of them.http://www.mdpi.com/2076-0817/3/3/499nucleotide sequencingrapid identificationfoodborne disease
collection DOAJ
language English
format Article
sources DOAJ
author Irshad M. Sulaiman
Emily Jacobs
Steven Simpson
Khalil Kerdahi
spellingShingle Irshad M. Sulaiman
Emily Jacobs
Steven Simpson
Khalil Kerdahi
Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region
Pathogens
nucleotide sequencing
rapid identification
foodborne disease
author_facet Irshad M. Sulaiman
Emily Jacobs
Steven Simpson
Khalil Kerdahi
author_sort Irshad M. Sulaiman
title Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region
title_short Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region
title_full Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region
title_fullStr Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region
title_full_unstemmed Molecular Identification of Isolated Fungi from Unopened Containers of Greek Yogurt by DNA Sequencing of Internal Transcribed Spacer Region
title_sort molecular identification of isolated fungi from unopened containers of greek yogurt by dna sequencing of internal transcribed spacer region
publisher MDPI AG
series Pathogens
issn 2076-0817
publishDate 2014-06-01
description In our previous study, we described the development of an internal transcribed spacer (ITS)1 sequencing method, and used this protocol in species-identification of isolated fungi collected from the manufacturing areas of a compounding company known to have caused the multistate fungal meningitis outbreak in the United States. In this follow-up study, we have analyzed the unopened vials of Greek yogurt from the recalled batch to determine the possible cause of microbial contamination in the product. A total of 15 unopened vials of Greek yogurt belonging to the recalled batch were examined for the detection of fungi in these samples known to cause foodborne illness following conventional microbiological protocols. Fungi were isolated from all of the 15 Greek yogurt samples analyzed. The isolated fungi were genetically typed by DNA sequencing of PCR-amplified ITS1 region of rRNA gene. Analysis of data confirmed all of the isolated fungal isolates from the Greek yogurt to be Rhizomucor variabilis. The generated ITS1 sequences matched 100% with the published sequences available in GenBank. In addition, these yogurt samples were also tested for the presence of five types of bacteria (Salmonella, Listeria, Staphylococcus, Bacillus and Escherichia coli) causing foodborne disease in humans, and found negative for all of them.
topic nucleotide sequencing
rapid identification
foodborne disease
url http://www.mdpi.com/2076-0817/3/3/499
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