A Bright and Colorful Future for G-Protein Coupled Receptor Sensors
Neurochemicals have a large impact on brain states and animal behavior but are notoriously hard to detect accurately in the living brain. Recently developed genetically encoded sensors obtained from engineering a circularly permuted green fluorescent protein into G-protein coupled receptors (GPCR) p...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2020-03-01
|
| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2020.00067/full |
| id |
doaj-22d5ccecba0846a08ac5f6014b244974 |
|---|---|
| record_format |
Article |
| spelling |
doaj-22d5ccecba0846a08ac5f6014b2449742020-11-25T02:30:46ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022020-03-011410.3389/fncel.2020.00067524260A Bright and Colorful Future for G-Protein Coupled Receptor SensorsLuca Ravotto0Loïc Duffet1Xuehan Zhou2Bruno Weber3Bruno Weber4Tommaso Patriarchi5Tommaso Patriarchi6Institute of Pharmacology and Toxicology, University of Zurich, Zurich, SwitzerlandInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, SwitzerlandInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, SwitzerlandInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, SwitzerlandNeuroscience Center Zurich, Zurich, SwitzerlandInstitute of Pharmacology and Toxicology, University of Zurich, Zurich, SwitzerlandNeuroscience Center Zurich, Zurich, SwitzerlandNeurochemicals have a large impact on brain states and animal behavior but are notoriously hard to detect accurately in the living brain. Recently developed genetically encoded sensors obtained from engineering a circularly permuted green fluorescent protein into G-protein coupled receptors (GPCR) provided a vital boost to neuroscience, by innovating the way we monitor neural communication. These new probes are becoming widely successful due to their flexible combination with state of the art optogenetic tools and in vivo imaging techniques, mainly fiber photometry and 2-photon microscopy, to dissect dynamic changes in brain chemicals with unprecedented spatial and temporal resolution. Here, we highlight current approaches and challenges as well as novel insights in the process of GPCR sensor development, and discuss possible future directions of the field.https://www.frontiersin.org/article/10.3389/fncel.2020.00067/fullneurotransmittersneuromodulatorsGPCRsfluorescent proteinsgenetically encoded sensorsin vivo imaging |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Luca Ravotto Loïc Duffet Xuehan Zhou Bruno Weber Bruno Weber Tommaso Patriarchi Tommaso Patriarchi |
| spellingShingle |
Luca Ravotto Loïc Duffet Xuehan Zhou Bruno Weber Bruno Weber Tommaso Patriarchi Tommaso Patriarchi A Bright and Colorful Future for G-Protein Coupled Receptor Sensors Frontiers in Cellular Neuroscience neurotransmitters neuromodulators GPCRs fluorescent proteins genetically encoded sensors in vivo imaging |
| author_facet |
Luca Ravotto Loïc Duffet Xuehan Zhou Bruno Weber Bruno Weber Tommaso Patriarchi Tommaso Patriarchi |
| author_sort |
Luca Ravotto |
| title |
A Bright and Colorful Future for G-Protein Coupled Receptor Sensors |
| title_short |
A Bright and Colorful Future for G-Protein Coupled Receptor Sensors |
| title_full |
A Bright and Colorful Future for G-Protein Coupled Receptor Sensors |
| title_fullStr |
A Bright and Colorful Future for G-Protein Coupled Receptor Sensors |
| title_full_unstemmed |
A Bright and Colorful Future for G-Protein Coupled Receptor Sensors |
| title_sort |
bright and colorful future for g-protein coupled receptor sensors |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Cellular Neuroscience |
| issn |
1662-5102 |
| publishDate |
2020-03-01 |
| description |
Neurochemicals have a large impact on brain states and animal behavior but are notoriously hard to detect accurately in the living brain. Recently developed genetically encoded sensors obtained from engineering a circularly permuted green fluorescent protein into G-protein coupled receptors (GPCR) provided a vital boost to neuroscience, by innovating the way we monitor neural communication. These new probes are becoming widely successful due to their flexible combination with state of the art optogenetic tools and in vivo imaging techniques, mainly fiber photometry and 2-photon microscopy, to dissect dynamic changes in brain chemicals with unprecedented spatial and temporal resolution. Here, we highlight current approaches and challenges as well as novel insights in the process of GPCR sensor development, and discuss possible future directions of the field. |
| topic |
neurotransmitters neuromodulators GPCRs fluorescent proteins genetically encoded sensors in vivo imaging |
| url |
https://www.frontiersin.org/article/10.3389/fncel.2020.00067/full |
| work_keys_str_mv |
AT lucaravotto abrightandcolorfulfutureforgproteincoupledreceptorsensors AT loicduffet abrightandcolorfulfutureforgproteincoupledreceptorsensors AT xuehanzhou abrightandcolorfulfutureforgproteincoupledreceptorsensors AT brunoweber abrightandcolorfulfutureforgproteincoupledreceptorsensors AT brunoweber abrightandcolorfulfutureforgproteincoupledreceptorsensors AT tommasopatriarchi abrightandcolorfulfutureforgproteincoupledreceptorsensors AT tommasopatriarchi abrightandcolorfulfutureforgproteincoupledreceptorsensors AT lucaravotto brightandcolorfulfutureforgproteincoupledreceptorsensors AT loicduffet brightandcolorfulfutureforgproteincoupledreceptorsensors AT xuehanzhou brightandcolorfulfutureforgproteincoupledreceptorsensors AT brunoweber brightandcolorfulfutureforgproteincoupledreceptorsensors AT brunoweber brightandcolorfulfutureforgproteincoupledreceptorsensors AT tommasopatriarchi brightandcolorfulfutureforgproteincoupledreceptorsensors AT tommasopatriarchi brightandcolorfulfutureforgproteincoupledreceptorsensors |
| _version_ |
1724828015418933248 |