High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis.
Reversed phase high pressure liquid chromatography (HPLC) solvent systems have been developed for the separation of intermediates in the formation of bile acids and bile acid conjugates from cholesterol. Four different mobile phases (water-methanol, 10 mM acetate buffer (pH 4.37)-methanol, 30 mM tri...
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Elsevier
1988-04-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520385266 |
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doaj-22b0b2ced59b4a2ca0e499607975a9002021-04-25T04:19:41ZengElsevierJournal of Lipid Research0022-22751988-04-01294532537High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis.K Prydz0B F Kase1J I Pedersen2Institute for Nutrition Research, School of Medicine, University of Oslo, Norway.Institute for Nutrition Research, School of Medicine, University of Oslo, Norway.Institute for Nutrition Research, School of Medicine, University of Oslo, Norway.Reversed phase high pressure liquid chromatography (HPLC) solvent systems have been developed for the separation of intermediates in the formation of bile acids and bile acid conjugates from cholesterol. Four different mobile phases (water-methanol, 10 mM acetate buffer (pH 4.37)-methanol, 30 mM trifluoroacetic acid (pH 2.9 with triethylamine)-methanol, and 50 mM potassium phosphate buffer (pH 7.0)-2-propanol) have been applied to obtain separation of all the main intermediates with use of the same reversed phase column (Zorbax ODS).http://www.sciencedirect.com/science/article/pii/S0022227520385266 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
K Prydz B F Kase J I Pedersen |
| spellingShingle |
K Prydz B F Kase J I Pedersen High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis. Journal of Lipid Research |
| author_facet |
K Prydz B F Kase J I Pedersen |
| author_sort |
K Prydz |
| title |
High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis. |
| title_short |
High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis. |
| title_full |
High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis. |
| title_fullStr |
High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis. |
| title_full_unstemmed |
High pressure liquid chromatography solvent systems for studies of bile acid biosynthesis. |
| title_sort |
high pressure liquid chromatography solvent systems for studies of bile acid biosynthesis. |
| publisher |
Elsevier |
| series |
Journal of Lipid Research |
| issn |
0022-2275 |
| publishDate |
1988-04-01 |
| description |
Reversed phase high pressure liquid chromatography (HPLC) solvent systems have been developed for the separation of intermediates in the formation of bile acids and bile acid conjugates from cholesterol. Four different mobile phases (water-methanol, 10 mM acetate buffer (pH 4.37)-methanol, 30 mM trifluoroacetic acid (pH 2.9 with triethylamine)-methanol, and 50 mM potassium phosphate buffer (pH 7.0)-2-propanol) have been applied to obtain separation of all the main intermediates with use of the same reversed phase column (Zorbax ODS). |
| url |
http://www.sciencedirect.com/science/article/pii/S0022227520385266 |
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AT kprydz highpressureliquidchromatographysolventsystemsforstudiesofbileacidbiosynthesis AT bfkase highpressureliquidchromatographysolventsystemsforstudiesofbileacidbiosynthesis AT jipedersen highpressureliquidchromatographysolventsystemsforstudiesofbileacidbiosynthesis |
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