The Interaction of Sheep Genomic DNA with a Cobalt(II) Complex Containing p-Nitrobenzoate and N,N`-Diethylnicotinamide Ligands

• Main Authors: Hacali Necefoglu, A. Kadir Devrim, Ali Arslantas
• Format: Article
• Language: English
• Published: MDPI AG 2007-12-01
• Series: International Journal of Molecular Sciences
• Subjects: DNA-metal interaction; Cobalt(II) complex; Sheep genomic DNA; pnitrobenzoate; N; N`-Diethylnicotinamid
• Online Access: http://www.mdpi.com/1422-0067/8/12/1225/

The synthesized cobalt(II) complex, CoPNBDENA and the binding of thiscomplex with sheep genomic DNA were investigated by UV–Visible absorption andviscosity techniques. Also the interaction of sheep genomic DNA with the complex wasstudied using the agarose gel electrophoresis method. The results indicated that thecomplex interacted with DNA. The nature of the binding seemed to be mainly anelectrostatic interaction between DNA and the cobalt(II) complex. Other binding modessuch as hydrogen bonds may also exist in this system. In this study, after the interaction ofDNA– CoPNBDENA, it was observed that the migration of the DNA band became slow asthe amount of cobalt(II) complex was increased. This clearly demonstrates that theCoPNBDENA complex neutralizes the negative charges of DNA.