POLYMORPHISM AND INTERSPECIFIC VARIABILITY OF CYTOCHROME OXYDASE SUBUNIT I (COI) GENE NUCLEOTIDE SEQUENCE IN SIBLING SPECIES of A AND B Anopheles messeae AND An. beklemishevi (DIPTERA: CULICIDAE)
Species identification of159 individuals of the Anopheles maculipennis complex from 5 localities ofUral andSiberia was conductedbyPCR-RFLP ITS2. The DNA sequences ofa COI gene fragment were determinedin 124 individuals from the localities. The Anopheles messeae groupCOI sequences were comparedwithth...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Institute of Cytology and Genetics of Siberian Branch of the Russian Academy of Sciences
2014-12-01
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| Series: | Vavilovskij Žurnal Genetiki i Selekcii |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/52 |
| Summary: | Species identification of159 individuals of the Anopheles maculipennis complex from 5 localities ofUral andSiberia was conductedbyPCR-RFLP ITS2. The DNA sequences ofa COI gene fragment were determinedin 124 individuals from the localities. The Anopheles messeae groupCOI sequences were comparedwiththose from a DNA database. VariabilityofCOI nucleotide sequences (measures H andπ) ofthe crypticspecies A andB Anopheles messeae andAn. beklemishevi was investigatedandquantitativelyestimated. It was foundthat the COI variabilitydidnot allow discrimination ofthe crypticspecies A andB Anopheles messeae. Neither didit show anygeographicregularityfor these species. It is concludedthat the COI polymorphism ofthe A andB Anopheles messeae species is common for them, as well as the chromosomal inversion polymorphism. It arose long before their divergence. The diagnosticimportance ofinterspecificITS2 variabilityis confirmed. |
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| ISSN: | 2500-0462 2500-3259 |