VIABILITY OF MAMMARY BOVINE LEUKOCYTES CHALLENGED WITH ANTI-MASTITIS DRUGS

• Main Authors: Camila Freitas Batista, Renata Caminha Gomes, Maiara Garcia Blagitz, Alice Maria Melville Paiva Della Libera
• Format: Article
• Language: English
• Published: Universidade Federal de Goiás 2017-02-01
• Series: Ciência Animal Brasileira
• Subjects: cow, cytotoxicity, milk cell, mammary gland.
• Online Access: https://www.revistas.ufg.br/vet/article/view/37893
• ISSN: 1518-2797; 1809-6891

The occurrence of viable cells in mammary gland secretions attests the functionality of the defense system. This fact, along with applied medicine, is fundamental for the healing udder . The objective of this study was to evaluate the in vitro viability of milk leukocytes challenged with four intramammary anti-mastitis drugs. We collected eight cow milk samples that were negative after a bacteriological examination. Cell suspensions were submitted to an evaluation of viability through Trypan Blue exclusion before and after incubation with the drugs. Mean pre-incubation viability rate (± standard deviation) of milk cells was 50.17% (± 6.50). Mean post-incubation viability rates (± standard deviation) of control group (devoid of any drug), Drug 1 (anhydrous cephalonium), Drug 2 (gentamicin), Drug 3 (procaine benzylpenicillin associated with dihydrostreptomycin nafcillin), and Drug 4 (benzathine cloxacillin) were, respectively, 31.36% (± 3.61); 26.23% (± 4.49); 7.82% (± 2.33); 3.10% (± 0.94); and 30.33% (± 4.14). The uppermost impairments were found when milk cells were challenged with Drug 2 and Drug 3. Although antimicrobial activity of tested drugs was not appraised, and a single aspect of cell function was tested, we concluded that treatments 2 and 3 could impair the functionality of milk leukocytes and could provide an ineffective immunological response, and an effective response is essential to control mammary infections. Keywords: cow; cell cytotoxicity; mammary gland; milk.