A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple Rhodopsins

Drosophila third-instar larvae exhibit changes in their behavioral responses to gravity and food as they transition from feeding to wandering stages. Using a thermal gradient encompassing the comfortable range (18°C to 28°C), we found that third-instar larvae exhibit a dramatic shift in thermal pref...

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Main Authors:	Takaaki Sokabe, Hsiang-Chin Chen, Junjie Luo, Craig Montell
Format:	Article
Language:	English
Published:	Elsevier 2016-10-01
Series:	Cell Reports
Subjects:	opsin rhodopsin TRP channel TRPA1 temperature sensation thermotaxis Drosophila larvae
Online Access:	http://www.sciencedirect.com/science/article/pii/S2211124716312505

id	doaj-22687c65f2624a94811ad4535e8f25c0
record_format	Article
spelling	doaj-22687c65f2624a94811ad4535e8f25c02020-11-25T01:17:13ZengElsevierCell Reports2211-12472016-10-0117233634410.1016/j.celrep.2016.09.028A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple RhodopsinsTakaaki Sokabe0Hsiang-Chin Chen1Junjie Luo2Craig Montell3Neuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USANeuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USANeuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USANeuroscience Research Institute and Department of Molecular, Cellular and Developmental Biology, University of California, Santa Barbara, Santa Barbara, CA 93106, USADrosophila third-instar larvae exhibit changes in their behavioral responses to gravity and food as they transition from feeding to wandering stages. Using a thermal gradient encompassing the comfortable range (18°C to 28°C), we found that third-instar larvae exhibit a dramatic shift in thermal preference. Early third-instar larvae prefer 24°C, which switches to increasingly stronger biases for 18°C–19°C in mid- and late-third-instar larvae. Mutations eliminating either of two rhodopsins, Rh5 and Rh6, wiped out these age-dependent changes in thermal preference. In larvae, Rh5 and Rh6 are thought to function exclusively in the light-sensing Bolwig organ. However, the Bolwig organ was dispensable for the thermal preference. Rather, Rh5 and Rh6 were required in trpA1-expressing neurons in the brain, ventral nerve cord, and body wall. Because Rh1 contributes to thermal selection in the comfortable range during the early to mid-third-instar stage, fine thermal discrimination depends on multiple rhodopsins.http://www.sciencedirect.com/science/article/pii/S2211124716312505opsinrhodopsinTRP channelTRPA1temperature sensationthermotaxisDrosophilalarvae
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Takaaki Sokabe Hsiang-Chin Chen Junjie Luo Craig Montell
spellingShingle	Takaaki Sokabe Hsiang-Chin Chen Junjie Luo Craig Montell A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple Rhodopsins Cell Reports opsin rhodopsin TRP channel TRPA1 temperature sensation thermotaxis Drosophila larvae
author_facet	Takaaki Sokabe Hsiang-Chin Chen Junjie Luo Craig Montell
author_sort	Takaaki Sokabe
title	A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple Rhodopsins
title_short	A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple Rhodopsins
title_full	A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple Rhodopsins
title_fullStr	A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple Rhodopsins
title_full_unstemmed	A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple Rhodopsins
title_sort	switch in thermal preference in drosophila larvae depends on multiple rhodopsins
publisher	Elsevier
series	Cell Reports
issn	2211-1247
publishDate	2016-10-01
description	Drosophila third-instar larvae exhibit changes in their behavioral responses to gravity and food as they transition from feeding to wandering stages. Using a thermal gradient encompassing the comfortable range (18°C to 28°C), we found that third-instar larvae exhibit a dramatic shift in thermal preference. Early third-instar larvae prefer 24°C, which switches to increasingly stronger biases for 18°C–19°C in mid- and late-third-instar larvae. Mutations eliminating either of two rhodopsins, Rh5 and Rh6, wiped out these age-dependent changes in thermal preference. In larvae, Rh5 and Rh6 are thought to function exclusively in the light-sensing Bolwig organ. However, the Bolwig organ was dispensable for the thermal preference. Rather, Rh5 and Rh6 were required in trpA1-expressing neurons in the brain, ventral nerve cord, and body wall. Because Rh1 contributes to thermal selection in the comfortable range during the early to mid-third-instar stage, fine thermal discrimination depends on multiple rhodopsins.
topic	opsin rhodopsin TRP channel TRPA1 temperature sensation thermotaxis Drosophila larvae
url	http://www.sciencedirect.com/science/article/pii/S2211124716312505
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A Switch in Thermal Preference in Drosophila Larvae Depends on Multiple Rhodopsins

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