Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism
Summary: Human dendritic cells were differentiated from blood monocytes and treated with malondialdehyde (MDA) conjugated with human serum albumin (HSA). Autologous T cells from human plaques or blood were co-cultured with the pre-treated dendritic cells or treated directly. MDA modifications were s...
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Elsevier
2019-08-01
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| Series: | JACC: Basic to Translational Science |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452302X19300853 |
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doaj-2254b1aea42345f48269e52ef050adf92020-11-25T01:27:28ZengElsevierJACC: Basic to Translational Science2452-302X2019-08-0144480494Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated MechanismMizanur Rahman, PhD0Johnny Steuer, MD, PhD1Peter Gillgren, MD, PhD2Ákos Végvári, PhD3Anquan Liu, MD, PhD4Johan Frostegård, MD, PhD5Institute of Environmental Medicine, Karolinska Institutet, Stockholm, SwedenSection of Vascular Surgery, Department of Surgery, Södersjukhuset, Institution of Clinical Science and Education, Karolinska Institutet, Stockholm, SwedenSection of Vascular Surgery, Department of Surgery, Södersjukhuset, Institution of Clinical Science and Education, Karolinska Institutet, Stockholm, SwedenDivision of Chemistry I, Department of Medical Biochemistry and Biophysics, Biomedicum, Karolinska Institutet, Stockholm, SwedenInstitute of Environmental Medicine, Karolinska Institutet, Stockholm, SwedenInstitute of Environmental Medicine, Karolinska Institutet, Stockholm, Sweden; Address for correspondence: Dr. Johan Frostegård, Unit of Immunology and Chronic Disease, Institute of Environmental Medicine, Karolinska Institutet, Nobels väg 13, 171 65 Stockholm, Sweden.Summary: Human dendritic cells were differentiated from blood monocytes and treated with malondialdehyde (MDA) conjugated with human serum albumin (HSA). Autologous T cells from human plaques or blood were co-cultured with the pre-treated dendritic cells or treated directly. MDA modifications were studied by mass spectrometry. MDA-HSA induced a pro-inflammatory DC-mediated T-cell activation and also a strong direct effect on T cells, inhibited by an inhibitor of oxidative stress and antibodies against MDA. Atherogenic heat shock protein-60 was strongly induced in T cells activated by MDA-HSA. Two peptide modifications in atherosclerotic patients' HSA were similar to those present in in vitro MDA-modified HSA. Key Words: atherosclerosis, dendritic cells, T cells, malondialdehyde, oxidized low-density lipoproteinhttp://www.sciencedirect.com/science/article/pii/S2452302X19300853 |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Mizanur Rahman, PhD Johnny Steuer, MD, PhD Peter Gillgren, MD, PhD Ákos Végvári, PhD Anquan Liu, MD, PhD Johan Frostegård, MD, PhD |
| spellingShingle |
Mizanur Rahman, PhD Johnny Steuer, MD, PhD Peter Gillgren, MD, PhD Ákos Végvári, PhD Anquan Liu, MD, PhD Johan Frostegård, MD, PhD Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism JACC: Basic to Translational Science |
| author_facet |
Mizanur Rahman, PhD Johnny Steuer, MD, PhD Peter Gillgren, MD, PhD Ákos Végvári, PhD Anquan Liu, MD, PhD Johan Frostegård, MD, PhD |
| author_sort |
Mizanur Rahman, PhD |
| title |
Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism |
| title_short |
Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism |
| title_full |
Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism |
| title_fullStr |
Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism |
| title_full_unstemmed |
Malondialdehyde Conjugated With Albumin Induces Pro-Inflammatory Activation of T Cells Isolated From Human Atherosclerotic Plaques Both Directly and Via Dendritic Cell–Mediated Mechanism |
| title_sort |
malondialdehyde conjugated with albumin induces pro-inflammatory activation of t cells isolated from human atherosclerotic plaques both directly and via dendritic cell–mediated mechanism |
| publisher |
Elsevier |
| series |
JACC: Basic to Translational Science |
| issn |
2452-302X |
| publishDate |
2019-08-01 |
| description |
Summary: Human dendritic cells were differentiated from blood monocytes and treated with malondialdehyde (MDA) conjugated with human serum albumin (HSA). Autologous T cells from human plaques or blood were co-cultured with the pre-treated dendritic cells or treated directly. MDA modifications were studied by mass spectrometry. MDA-HSA induced a pro-inflammatory DC-mediated T-cell activation and also a strong direct effect on T cells, inhibited by an inhibitor of oxidative stress and antibodies against MDA. Atherogenic heat shock protein-60 was strongly induced in T cells activated by MDA-HSA. Two peptide modifications in atherosclerotic patients' HSA were similar to those present in in vitro MDA-modified HSA. Key Words: atherosclerosis, dendritic cells, T cells, malondialdehyde, oxidized low-density lipoprotein |
| url |
http://www.sciencedirect.com/science/article/pii/S2452302X19300853 |
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