OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice

OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice

Rice (Oryza sativa L.) is a major food crop worldwide. Plant height and yield are important agronomic traits of rice. Several genes regulating plant height and/or yield have been cloned. However, the molecular mechanisms coordinating plant height and yield are not fully characterized. Here, we repor...

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Main Authors: Yahui Liu, Yu Zhu, Xuding Xu, Fan Sun, Jingshui Yang, Liming Cao, Xiaojin Luo
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2019-10-01
Series:Crop Journal
Online Access:http://www.sciencedirect.com/science/article/pii/S2214514119300467
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spelling doaj-222bf8ea312041abb860526fe569b5722021-02-02T05:25:42ZengKeAi Communications Co., Ltd.Crop Journal2214-51412019-10-0175707714OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in riceYahui Liu0Yu Zhu1Xuding Xu2Fan Sun3Jingshui Yang4Liming Cao5Xiaojin Luo6State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, ChinaState Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, ChinaInstitute of Crop Breeding and Cultivation, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China; Corresponding author.State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China; Engineering Research Center of Gene Technology, Ministry of Education, Fudan University, Shanghai 200438, China; Correspondence to: X. Luo, State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Sciences, Fudan University, Shanghai 200438, China.Rice (Oryza sativa L.) is a major food crop worldwide. Plant height and yield are important agronomic traits of rice. Several genes regulating plant height and/or yield have been cloned. However, the molecular mechanisms coordinating plant height and yield are not fully characterized. Here, we report a novel gene, OstMAPKKK5 that encodes a truncated variant of a mitogen-activated protein kinase kinase kinase 5 (OsMAPKKK5) lacking an intact kinase domain. Transgenic plants overexpressing OstMAPKKK5 in indica cultivar 9311 showed increased plant height, grain length, grain width, 1000-grain weight, grain number per main panicle, and yield. Real-time quantitative PCR showed that OstMAPKKK5 was widely expressed in various tissues and developmental stages. The increased plant height and yield were attributed to enlarged cell size. Overexpression of OstMAPKKK5 led to higher contents of various forms of endogenous gibberellin (GA), especially the most common active forms, GA1, GA3, GA4. We concluded that OstMAPKKK5 positively regulates plant height and yield in rice by affecting cell size, and that its underlying mechanism is based on increased endogenous GA content. Keywords: OstMAPKKK5, Plant height and yield, Cell size, Gibberellin, Oryza sativahttp://www.sciencedirect.com/science/article/pii/S2214514119300467
collection DOAJ
language English
format Article
sources DOAJ
author Yahui Liu
Yu Zhu
Xuding Xu
Fan Sun
Jingshui Yang
Liming Cao
Xiaojin Luo
spellingShingle Yahui Liu
Yu Zhu
Xuding Xu
Fan Sun
Jingshui Yang
Liming Cao
Xiaojin Luo
OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice
Crop Journal
author_facet Yahui Liu
Yu Zhu
Xuding Xu
Fan Sun
Jingshui Yang
Liming Cao
Xiaojin Luo
author_sort Yahui Liu
title OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice
title_short OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice
title_full OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice
title_fullStr OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice
title_full_unstemmed OstMAPKKK5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice
title_sort ostmapkkk5, a truncated mitogen-activated protein kinase kinase kinase 5, positively regulates plant height and yield in rice
publisher KeAi Communications Co., Ltd.
series Crop Journal
issn 2214-5141
publishDate 2019-10-01
description Rice (Oryza sativa L.) is a major food crop worldwide. Plant height and yield are important agronomic traits of rice. Several genes regulating plant height and/or yield have been cloned. However, the molecular mechanisms coordinating plant height and yield are not fully characterized. Here, we report a novel gene, OstMAPKKK5 that encodes a truncated variant of a mitogen-activated protein kinase kinase kinase 5 (OsMAPKKK5) lacking an intact kinase domain. Transgenic plants overexpressing OstMAPKKK5 in indica cultivar 9311 showed increased plant height, grain length, grain width, 1000-grain weight, grain number per main panicle, and yield. Real-time quantitative PCR showed that OstMAPKKK5 was widely expressed in various tissues and developmental stages. The increased plant height and yield were attributed to enlarged cell size. Overexpression of OstMAPKKK5 led to higher contents of various forms of endogenous gibberellin (GA), especially the most common active forms, GA1, GA3, GA4. We concluded that OstMAPKKK5 positively regulates plant height and yield in rice by affecting cell size, and that its underlying mechanism is based on increased endogenous GA content. Keywords: OstMAPKKK5, Plant height and yield, Cell size, Gibberellin, Oryza sativa
url http://www.sciencedirect.com/science/article/pii/S2214514119300467
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