Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests

Competition between bound and free peptides in an ELISA-based procedure that assays peptides derived from protein digests

<p>Abstract</p> <p>Background</p> <p>We describe an ELISA-based method that can be used to identify and quantitate proteins in biological samples. In this method, peptides in solution, derived from proteolytic digests of the sample, compete with substrate-attached synth...

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Bibliographic Details
Main Authors: Pace Umberto, Bishara-Shieban Janette, Glickstein Hava, Braitbard Ori, Stein Wilfred D
Format: Article
Language:English
Published: BMC 2006-05-01
Series:Proteome Science
Online Access:http://www.proteomesci.com/content/4/1/12

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