Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry

Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry

The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are kerati...

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Main Authors: Kateřina Mikulíková, Oleg Romanov, Ivan Miksik, Adam Eckhardt, Statis Pataridis, Pavla Sedláková
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/759604
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spelling doaj-220541dc3219412688327c07fe79f8692020-11-24T21:32:32ZengHindawi LimitedThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/759604759604Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass SpectrometryKateřina Mikulíková0Oleg Romanov1Ivan Miksik2Adam Eckhardt3Statis Pataridis4Pavla Sedláková5Institute of Physiology, Academy of Science of the Czech Republic, VÍdeňská 1083, Prague, Czech RepublicKalmykian State University, Elista, RussiaInstitute of Physiology, Academy of Science of the Czech Republic, VÍdeňská 1083, Prague, Czech RepublicInstitute of Physiology, Academy of Science of the Czech Republic, VÍdeňská 1083, Prague, Czech RepublicInstitute of Physiology, Academy of Science of the Czech Republic, VÍdeňská 1083, Prague, Czech RepublicInstitute of Physiology, Academy of Science of the Czech Republic, VÍdeňská 1083, Prague, Czech RepublicThe saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α1) (from bovine) and collagen type I (α2) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.http://dx.doi.org/10.1100/2012/759604
collection DOAJ
language English
format Article
sources DOAJ
author Kateřina Mikulíková
Oleg Romanov
Ivan Miksik
Adam Eckhardt
Statis Pataridis
Pavla Sedláková
spellingShingle Kateřina Mikulíková
Oleg Romanov
Ivan Miksik
Adam Eckhardt
Statis Pataridis
Pavla Sedláková
Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
The Scientific World Journal
author_facet Kateřina Mikulíková
Oleg Romanov
Ivan Miksik
Adam Eckhardt
Statis Pataridis
Pavla Sedláková
author_sort Kateřina Mikulíková
title Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_short Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_full Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_fullStr Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_full_unstemmed Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry
title_sort study of saiga horn using high-performance liquid chromatography with mass spectrometry
publisher Hindawi Limited
series The Scientific World Journal
issn 1537-744X
publishDate 2012-01-01
description The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α1) (from bovine) and collagen type I (α2) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.
url http://dx.doi.org/10.1100/2012/759604
work_keys_str_mv AT katerinamikulikova studyofsaigahornusinghighperformanceliquidchromatographywithmassspectrometry
AT olegromanov studyofsaigahornusinghighperformanceliquidchromatographywithmassspectrometry
AT ivanmiksik studyofsaigahornusinghighperformanceliquidchromatographywithmassspectrometry
AT adameckhardt studyofsaigahornusinghighperformanceliquidchromatographywithmassspectrometry
AT statispataridis studyofsaigahornusinghighperformanceliquidchromatographywithmassspectrometry
AT pavlasedlakova studyofsaigahornusinghighperformanceliquidchromatographywithmassspectrometry
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