Study of Saiga Horn Using High-Performance Liquid Chromatography with Mass Spectrometry

The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are kerati...

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Bibliographic Details
Main Authors: Kateřina Mikulíková, Oleg Romanov, Ivan Miksik, Adam Eckhardt, Statis Pataridis, Pavla Sedláková
Format: Article
Language:English
Published: Hindawi Limited 2012-01-01
Series:The Scientific World Journal
Online Access:http://dx.doi.org/10.1100/2012/759604
Description
Summary:The saiga horns have been investigated the using of modern analytic methods. High-performance liquid chromatography (HPLC) with mass-spectrometric (MS and MS/MS) detection and polyacrylamide gel electrophoresis (PAGE) were used. It could be concluded that basic proteins of the saiga horns are keratins and collagen. The basic representation protein in all samples is keratin type I microfibrillar (from sheep), keratin type II microfibrillar (from sheep), collagen type I (α1) (from bovine) and collagen type I (α2) (from bovine). Free amino acids we determined in all samples are nontreated by enzyme.
ISSN:1537-744X