In Vitro and In Vivo Evaluation of Alexa Fluor 680-Bombesin[7–14]NH Peptide Conjugate, a High-Affinity Fluorescent Probe with High Selectivity for the Gastrin-Releasing Peptide Receptor

Gastrin-releasing peptide (GRP) receptors are overexpressed on several types of human cancer cells, including breast, prostate, small cell lung, and pancreatic cancers. Bombesin (BBN), a 14–amino acid peptide that is an analogue of human GRP, binds to GRP receptors with very high affinity and specif...

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Bibliographic Details
Main Authors: Lixin Ma, Ping Yu, Bhadrasetty Veerendra, Tammy L. Rold, Lauren Retzloff, Adam Prasanphanich, Gary Sieckman, Timothy J. Hoffman, Wynn A. Volkert, Charles J. Smith
Format: Article
Language:English
Published: Hindawi - SAGE Publishing 2007-05-01
Series:Molecular Imaging
Online Access:https://doi.org/10.2310/7290.2007.00013
Description
Summary:Gastrin-releasing peptide (GRP) receptors are overexpressed on several types of human cancer cells, including breast, prostate, small cell lung, and pancreatic cancers. Bombesin (BBN), a 14–amino acid peptide that is an analogue of human GRP, binds to GRP receptors with very high affinity and specificity. The aim of this study was to develop a new fluorescent probe based on BBN having high tumor uptake and optimal pharmacokinetics for specific targeting and optical imaging of human breast cancer tissue. In this study, solid-phase peptide synthesis was used to produce H 2 N-glycylglycylglycine-BBN[7–14]NH 2 peptide with the following general sequence: H 2 N-G-G-G-Q-W-A-V-G-H-L-M-(NH 2 ). This conjugate was purified by reversed-phase high-performance liquid chromatography and characterized by electrospray-ionization mass spectra. The fluorescent probe Alexa Fluor 680-G-G-G-BBN[7–14]NH 2 conjugate was prepared by reaction of Alexa Fluor 680 succinimidyl ester to H 2 N-G-G-G-BBN[7–14]NH 2 in dimethylformamide (DMF). In vitro competitive binding assays, using 125 I-Tyr 4 -BBN as the radiolabeling gold standard, demonstrated an inhibitory concentration 50% value of 7.7 ± 1.4 nM in human T-47D breast cancer cells. Confocal fluorescence microscopy images of Alexa Fluor 680-G-G-G-BBN[7–14]NH 2 in human T-47D breast cancer cells indicated specific uptake, internalization, and receptor blocking of the fluorescent bioprobe in vitro. In vivo investigations in SCID mice bearing xenografted T-47D breast cancer lesions demonstrated the ability of this new conjugate to specifically target tumor tissue with high selectivity and affinity.
ISSN:1536-0121