A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR

A simple method for extracting DNA from rhododendron plants infected with Phytophthora spp. for use in PCR

Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora...

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Bibliographic Details
Main Authors: Trzewik Aleksandra, Nowak Katarzyna J., Orlikowska Teresa
Format: Article
Language:English
Published: Polish Academy of Sciences 2016-01-01
Series:Journal of Plant Protection Research
Subjects:
conventional PCR
detection
infected rhododendron leaves
Phytophthora species
real-time PCR
total DNA extraction
Online Access:http://www.degruyter.com/view/j/jppr.2016.56.issue-1/jppr-2016-0014/jppr-2016-0014.xml?format=INT
Description
Summary:Among the numerous protocols that describe the extraction of DNA, those relating to the isolation of DNA from infected plants, are rare. This study describes a rapid and reliable method of extracting a high quality and quantity of DNA from rhododendron leaves artificially infected with Phytophthora cactorum, P. cambivora, P. cinnamomi, P. citrophthora, and P. plurivora. The use of the modified Doyle and Doyle protocol (1987) allowed us to obtain high quantity and quality DNA (18.26 μg from 100 mg of the fresh weight of infected leaves at the ratios of A260/280 and A260/230 - 1.83 and 1.72, respectively), suitable for conventional polymerase chain reaction (PCR) and real-time PCR amplifications.
ISSN:1899-007X

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