Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by In Vivo Periplasmic Forster Resonance Energy Transfer Assay in Escherichia coli

Activity-Related Conformational Changes in d,d-Carboxypeptidases Revealed by In Vivo Periplasmic Forster Resonance Energy Transfer Assay in Escherichia coli

One of the mechanisms of β-lactam antibiotic resistance requires the activity of d,d-carboxypeptidases (d,d-CPases) involved in peptidoglycan (PG) synthesis, making them putative targets for new antibiotic development. The activity of PG-synthesizing enzymes is often correlated with their associatio...

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Bibliographic Details
Main Authors: Nils Y. Meiresonne, René van der Ploeg, Mark A. Hink, Tanneke den Blaauwen, Lotte Søgaard-Andersen
Format: Article
Language:English
Published: American Society for Microbiology 2017-09-01
Series:mBio
Online Access:http://mbio.asm.org/cgi/content/full/8/5/e01089-17

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