2D- and 3D-Based Intestinal Stem Cell Cultures for Personalized Medicine

• Main Authors: Yuan Liu, Ye-Guang Chen
• Format: Article
• Language: English
• Published: MDPI AG 2018-11-01
• Series: Cells
• Subjects: intestinal stem cells; organoids; monolayer; cell culture; personalized medicine
• Online Access: https://www.mdpi.com/2073-4409/7/12/225

Colorectal cancer (CRC) is one of the most common cancers that have high occurrence and death in both males and females. As various factors have been found to contribute to CRC development, personalized therapies are critical for efficient treatment. To achieve this purpose, the establishment of patient-derived tumor models is critical for diagnosis and drug test. The establishment of three-dimensional (3D) organoid cultures and two-dimensional (2D) monolayer cultures of patient-derived epithelial tissues is a breakthrough for expanding living materials for later use. This review provides an overview of the different types of 2D- and 3D-based intestinal stem cell cultures, their potential benefits, and the drawbacks in personalized medicine in treatment of the intestinal disorders.