Application of a cost-effective DNA extraction protocol for screening transgenic and CRISPR-edited primary goat cells

Application of a cost-effective DNA extraction protocol for screening transgenic and CRISPR-edited primary goat cells

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The genotyping of genetically-modified cells is a crucial step in studies of transgenics and genomic editing with systems such as CRISPR/Cas. The detection of genome editing events can be directly related to the genotyping methodology used, which is influenced by its costs, since many experiments re...

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Bibliographic Details
Main Authors: Louhanna Pinheiro Rodrigues Teixeira, Francisco Eder de Moura Lopes, André Saraiva Leão Marcelo Antunes, Matheus Soares Alves, André Marrocos Miranda, Saul Gaudencio Neto, Leonardo Tondello Martins, Ana Cristina de Oliveira Monteiro Moreira, Kaio Cesar Simiano Tavares, René Massimiliano Marsano
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2020-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7500585/?tool=EBI

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